Cross reactivity between many anti-human antibodies for their hamster homologs provide the tools to study the signal transduction pathway activated by asbestos and SV40 in the malignant mesothelioma model

Our plain-English summary of Cross reactivity between many anti-human antibodies for their hamster homologs provide the tools to study the signal transduction pathway activated by asbestos and SV40 in the malignant mesothelioma model, by B. Kroczynska, M. Carbone (Molecular Carcinogenesis, 2006).

The study

• Authors: B. Kroczynska, M. Carbone
• Journal: Molecular Carcinogenesis
• Published: 2006;45(7):537-42
• Cited by: 1 article, as counted by NIH iCite
• Read it: PubMed · Publisher

Abstract

The aim of this study was to test the possibility of using human antibodies to study the pathogenic mechanism of SV40 and asbestos in a hamster mesothelioma model. The cellular lysates from human and hamster primary mesothelial cells were tested by Western blot analysis. All of the antibodies we tested (HGF, Notch, VEGF, Sp1, p53, PP2A, p-ERK1, p-c-jun, Fra1, Fra2, MMP1, MMP9, NFkappaB p65, IkappaB, GAPDH) cross-reacted with their hamster counterparts. These data indicate that hamster mesothelioma model and more in general hamster experimental model, can be used for functional studies because many mouse, rabbit, and goat monoclonal antibodies prepared against human antigens cross-react with their hamster counterparts.

In plain English

This brief laboratory report tested whether commercial antibodies made against human proteins also recognize the matching hamster proteins. The aim was to make it easier to study a hamster model of malignant mesothelioma linked to asbestos and SV40 virus. The authors compared protein extracts from two sources using Western blotting: normal human mesothelial cells from donors without cancer, and mesothelial cells scraped from the pleura and peritoneum of normal Syrian golden hamsters. The article does not describe exposing these cells to asbestos or SV40. Every antibody tested, including those for p53, Sp1, VEGF, MMP1, MMP9 and NF-kB p65, detected its hamster counterpart. Compared with human cells, the signal in hamster cells was almost identical for Sp1 and similar for MMP1 and MMP9. It was slightly weaker for HGF, Notch-1 and VEGF, and significantly weaker for p53 and phosphorylated IkB. The authors concluded that antibodies against human proteins can be used to detect the hamster proteins in their hypothetical model of how mesothelioma develops. They also concluded that the human and hamster proteins appear very similar in level and size.

We wrote this plain-English summary from the full article with the help of AI, and a second AI model checked each sentence against the article. It is not the authors' wording; the abstract and the article are their own account.

What the study did and found

• Design: Laboratory study (brief communication) comparing human and hamster cell protein extracts by Western blot
• Who was studied: Primary mesothelial cell cultures from normal Syrian golden hamsters (pleura and peritoneum) and from human donors without cancer, used at passages 3 to 7; numbers of animals and donors not stated
• Exposure: No asbestos or SV40 exposure is described in the experiments. What was tested was whether antibodies raised against human proteins (HGF, Notch-1, VEGF, Sp1, p53, PP2A, ERK, c-jun, Fra1, Fra2, MMP1, MMP9, NF-kB p65, IkB, GAPDH) recognize hamster proteins.
• Main results:
  • Human antibodies detected HGF, Notch-1 and VEGF in hamster cell lysate, with a signal slightly less pronounced than in human cell lysates
  • Anti-Sp1 antibodies recognized hamster Sp1 with signal intensity almost identical to human mesothelial cells
  • Anti-p53 antibodies cross-reacted with hamster p53, but the signal was significantly stronger in human than in hamster lysate
  • Antibodies cross-reacted with hamster PP2A, ERK1/2, p-c-jun, Fra1 and Fra2
  • MMP1 and MMP9 antibody signals were similar in human and hamster cell lysates
  • NF-kB p65 and IkB were detected in hamster cells; the phosphorylated IkB signal was significantly lower in hamster than in human lysate
• The authors' conclusion: The authors concluded that antibodies against human proteins can be used to detect the hamster proteins in their hypothetical model of mesothelioma development, and that human and hamster mesothelial cell proteins appear very similar in level and subunit size.

Topics

The National Library of Medicine indexes this study under subjects our site covers: Mesothelioma.

Medical terms in this study, explained in our Lexicon: SV40, Malignant Mesothelioma, Mesothelioma, Mesothelial Cells, Monoclonal.

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