Rapid and easy method for monitoring oxidative stress markers in body fluids of patients with asbestos or silica-induced lung diseases

Our plain-English summary of Rapid and easy method for monitoring oxidative stress markers in body fluids of patients with asbestos or silica-induced lung diseases, by K. Syslová, P. Kacer, M. Kuzma, et al (Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 2009).

The study

• Authors: K. Syslová, P. Kacer, M. Kuzma, V. Najmanová, Z. Fenclová, S. Vlcková, J. Lebedová, D. Pelclová
• Journal: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
• Published: 2009;877(24):2477-86
• Cited by: 57 articles, as counted by NIH iCite
• Read it: PubMed · Publisher

Abstract

Sensitive assay method was developed for a parallel, rapid and precise determination of the most prominent oxidative stress biomarkers: 8-iso-prostaglandin F(2alpha), malondialdehyde and 4-hydroxynonenal. The method consisted of a pre-treatment part a solid-phase extraction, for rapid and effective isolation of biomarkers from body fluids (exhaled breath condensate, plasma and urine) and the detection method LC-ESI-MS/MS, where the selected reaction monitoring mode was used for its extremely high degree of selectivity and the stable-isotope-dilution assay for its high precision of quantification. The developed method was characterized by the following parameters: the imprecision was below 14.3%, the mean inaccuracy was determined to be lower than 13.1%. The method was tested on samples obtained from patients diagnosed with asbestosis, pleural hyalinosis or silicosis, i.e. occupational lung diseases caused by fibrogenic dusts, inducing oxidative stress in the respiratory system, and then compared to samples from healthy subjects. The difference in concentration levels of biomarkers between the two groups was perceptible in all the body fluids (the difference observed in an exhaled breath condensate was statistically most significant).

In plain English

This laboratory study developed a method for measuring three chemical markers of oxidative stress, an imbalance between the body's production of reactive oxygen and its ability to detoxify it or repair the damage. The markers were 8-iso-prostaglandin F2-alpha, malondialdehyde (MDA) and 4-hydroxynonenal (HNE). They were measured in exhaled breath condensate (moisture from the breath), urine and blood plasma. The authors checked its precision and accuracy on prepared samples. They then tested it on 20 male non-smoking patients with asbestosis, pleural hyalinosis or silicosis, exposed at work to asbestos or silica dust for 24 years on average. These were compared with 10 healthy male non-smokers. In breath condensate, mean levels of all three markers were higher in patients, for example MDA 72 versus 45 ng/ml. The authors reported these differences as statistically significant. Mean urine and plasma levels were also higher in patients, but the article reported no P values or significance statement for these fluids. The article did not name the asbestos fiber types involved. The authors concluded that the method expands diagnostic potential for lung diseases involving oxidative stress.

We wrote this plain-English summary from the full article with the help of AI, and a second AI model checked each sentence against the article. It is not the authors' wording; the abstract and the article are their own account.

What the study did and found

• Design: Laboratory method development and validation study, tested in a small comparison of patients and healthy controls
• Who was studied: 20 male non-smoking patients with occupational lung disease (asbestosis, pleural hyalinosis or silicosis), average age 65 plus or minus 6 years. They were compared with 10 male non-smoking controls with no recorded occupational exposure to fibrogenic dusts, average age 63 plus or minus 5 years. Further study details are in a separate publication.
• Exposure: Previous occupational exposure to asbestos or silica dust, 24 years on average. Fiber types and products are not given.
• Main results:
  • Method validation: imprecision was below 14.3% and mean inaccuracy was lower than 13.1%.
  • Exhaled breath condensate, mean levels in patients versus controls: 8-iso-prostaglandin F2-alpha 71 versus 52 pg/ml; MDA 72 versus 45 ng/ml; HNE 233 versus 165 ng/ml.
  • The authors reported the differences in mean breath condensate levels of all three markers between patients and controls as statistically significant.
  • Urine, mean levels in patients versus controls (per mmol creatinine): 8-iso-prostaglandin F2-alpha 34 versus 25 ng; MDA 8.4 versus 6.8; HNE 296 versus 211. No urine-specific P value or significance statement was reported.
  • Plasma, mean levels in patients versus controls: 8-iso-prostaglandin F2-alpha 66 versus 46 pg/ml; MDA 373 versus 352 ng/ml; HNE 2032 versus 1736 ng/ml. No plasma-specific P value or significance statement was reported.
  • The authors noted that mild elevation of some markers in plasma and urine may be linked to systemic diseases in some subjects of both groups.
• The authors' conclusion: The authors concluded that measuring these markers in exhaled breath condensate (and possibly urine or plasma) expands diagnostic potential for lung diseases involving oxidative stress. They added that it may help understanding of how silicosis and asbestosis develop.

Topics

Medical terms in this study, explained in our Lexicon: Oxidative Stress, Biomarker, Silicosis, Occupational Lung Disease, Fibrogenic.

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