558 results for “Reaction Department”, page 10 of 28
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Medical study · Medical research · 1955
Local inflammatory response produced by solutions sterilized with asbestos type filters
…due to the presence of asbestos fibers in the injected filtrate. Further studies indicated that the eosinophilia was not merely the reaction to a non-specific irritating substance. Talc produced a response identical to the asbestos…
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Medical study · Medical research · 1985
Exogenous particles in lymph nodes in patients with shrinking pleuritis with atelectasis
…pleuritis with atelectasis is a disease in which a compression atelectasis of the lung is caused by shrinking of the inflamed visceral pleura. This reaction has been attributed to asbestos. By means of scanning electron microscopy…
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Medical study · Medical research · 1986
Distinct keratin patterns demonstrated by immunoperoxidase staining of adenocarcinomas, carcinoids, and mesotheliomas using polyclonal and monoclonal anti-keratin antibodies
…10 adenocarcinomas, 10 carcinoids, and 4 mesotheliomas by an immunoperoxidase reaction using 3 polyclonal and 3 monoclonal antibodies to keratin. When color development in diaminobenzidine (DAB) was allowed to proceed for less than 2 minutes, distinct…
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Medical study · Medical research · 1990
Mesothelial proliferation due to asbestos and man-made fibres. Experimental studies on rat omentum
…in rats has proven to be an appropriate method controlling fibrogenicity and carcinogenicity of asbestos fibres and other fibrous dusts. We analyzed the reaction patterns of mesothelial cover layer to different natural mineral fibres (crocidolite, chrysotile…
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Medical study · Medical research · 2013
Diagnostic usefulness of p16/CDKN2A FISH in distinguishing between sarcomatoid mesothelioma and fibrous pleuritis
…examination is inconclusive. We studied the usefulness of deletion of p16 with fluorescence in situ hybridization (FISH) and p16 hypermethylation with polymerase chain reaction for the diagnosis and prognosis of malignant pleural mesothelioma (MPM). We analyzed…
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Medical study · Medical research · 2010
Diagnosis and origin determination of malignant pleural effusions through the use of the breast cancer marker human mammaglobin
…be expressed in malignant pleural effusions (PEs), we investigated the relevance of hMAM reverse-transcriptase polymerase chain reaction (RT-PCR) for their diagnosis and determination of primary origin. Two hundred and twenty-eight malignant (132 male…
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Medical study · Medical research · 2011
Aromatase inhibitor exemestane has antiproliferative effects on human mesothelioma cells
…aromatase (CYP19A1) in malignant mesothelioma (MM). We found CYP19A1 in five human MM cell lines using reverse transcription polymerase chain reaction and Western immunoblots and in a group of samples from patients with MM by immunohistochemistry…
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Medical study · Medical research · 2010
Complete regression of human malignant mesothelioma xenografts following local injection of midkine promoter-driven oncolytic adenovirus
…the tumor-specific midkine (Mdk) promoter could confer transcriptional targeting to oncolytic adenoviruses for effective treatment of malignant mesothelioma. We analysed Mdk expression by quantitative reverse transcription-polymerase chain reaction in six human mesothelioma cell lines…
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Medical study · Medical research · 1986
Alveolar macrophage stimulation of lung fibroblast growth in asbestos-induced pulmonary fibrosis
…proliferation, and collagen deposition. To evaluate the potential involvement of alveolar macrophages in the subsequent fibrogenic reaction, the authors studied the effects of macrophages from normal and asbestos-treated rats upon lung fibroblast proliferation in vitro…
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Medical study · Medical research · 2010
Combined use of X-ray photoelectron and Mössbauer spectroscopic techniques in the analytical characterization of iron oxidation state in amphibole asbestos
…presence, structural coordination, and oxidation state of iron at the fiber surface are potentially important for the biological effects of asbestos because iron can catalyze the Haber-Weiss reaction, generating the reactive oxygen species OH. Literature…
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Medical study · Medical research · 2001
Absence of SV-40 large T antigen (Tag) in malignant mesothelioma effusions: an immunocytochemical study
…a function of a nuclear protein, the large T antigen (Tag). SV-40 Tag DNA sequences are detected by the polymerase chain reaction in 40-80% of malignant mesothelial proliferations. However, the role of immunohistochemistry (IHC…
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Medical study · Medical research · 2013
Novel cationic-modified salep as an efficient flocculating agent for settling of cement slurries
…and 3-(methacryloylamino)propyl]trimethylammonium chloride (MAPTAC) onto salep using free radical polymerization initiated by ammonium persulfate (APS) to produce cationic salep [S-g-P(AM-co-MAPTAC)]. Reaction parameters (monomers/salep ratio, concentration of reactants…
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Medical study · Medical research · 2021
MicroRNA expression in lung tissues of asbestos-exposed mice: Upregulation of miR-21 and downregulation of tumor suppressor genes Pdcd4 and Reck
…mice four times weekly. We extracted total RNA from lung tissues and performed microarray analysis for miRNA and gene expression. We also carried out real-time polymerase chain reaction (PCR), Western blotting, and immunohistochemistry to confirm…
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Medical study · Medical research · 2016
Functional Analysis of the Adrenomedullin Pathway in Malignant Pleural Mesothelioma
…protein 2 (CLR/RAMP2) and calcitonin receptor-like receptor/receptor activity-modifying protein 3 (CLR/RAMP3). Real time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) was used to assess the steady-state levels of AM…
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Medical study · Medical research · 2021
Osteopontin mRNA expression by rat mesothelial cells exposed to multi-walled carbon nanotubes as a potential biomarker of chronic neoplastic transformation in vitro
…were used as a positive control. Osteopontin (OPN) mRNA was assayed as a biomarker of transformation by real time quantitative polymerase chain reaction (qPCR) and transformation was determined by a cell invasion assay. Exposure to rMWCNTs…
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Medical study · Medical research · 2016
Young investigator challenge: MicroRNA-21/MicroRNA-126 profiling as a novel tool for the diagnosis of malignant mesothelioma in pleural effusion cytology
…authors evaluated the utility of an miRNA-based classifier as a complement to cytology. Quantitative reverse transcriptase-polymerase chain reaction analysis of 15 miRNAs was performed in mesothelial (MET-5A) and MM (H28 and H2052) cell…
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Medical study · Medical research · 2016
Low homozygous/high heterozygous deletion status by p16 FISH correlates with a better prognostic group than high homozygous deletion status in malignant pleural mesothelioma
…FISH was performed in 93 MPMs and 47 RMHs. Real-time polymerase chain reaction (PCR) and methylation specific PCR (MSP) were also performed for cases in which DNA was available. Overall survival (OS) was assessed via…
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Medical study · Medical research · 2020
Targeting ROR1 in combination with pemetrexed in malignant mesothelioma cells
…Microarray, quantitative polymerase chain reaction, and Western blot analyses showed that there were differences in the suppression of mRNA and proteins between H2452 and H28 cells transfected with siROR1 compared with those transfected with control siRNA…
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Medical study · Medical research · 2021
UHRF1 Is a Novel Druggable Epigenetic Target in Malignant Pleural Mesothelioma
…in malignant pleural mesothelioma (MPM). This study was undertaken to explore the therapeutic potential of targeting UHRF1 in MPM. Microarray, real-time quantitative reverse transcription-polymerase chain reaction, immunoblot, and immunohistochemistry techniques were used to evaluate…
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Medical study · Medical research · 2019
Clinical significance of serum DRAM1 mRNA, ARSA mRNA, hsa-miR-2053 and lncRNA-RP1-86D1.3 axis expression in malignant pleural mesothelioma
…miR-2053) and long noncoding RNA ( lncRNA-RP1-86D1.3). Then, quantitative real-time polymerase chain reaction (qPCR) validation in sera of 60 MPM patients, 20 chronic asbestos exposure patients, and 20 healthy volunteers was done…