145 results for “Reaction Department”, page 3 of 8
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Medical study · Medical research · 1991
Preparation of radiolabeled bioactive asbestos fibers
…were radiolabeled successfully. Mild reaction conditions and short reaction times were found under which approximately 90% of the added 68Ge and 68Ga bound to the fibers. The radiolabel was retained even after washing the fibers extensively…
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Medical study · Medical research · 1998
Asbestos fibers and interleukin-1 upregulate the formation of reactive nitrogen species in rat pleural mesothelial cells
…polymerase chain reaction (RT-PCR), increased progressively from 2 to 12 h in IL-1beta-containing cultures. Nitrite (NO2-), the stable oxidation product of .NO in mesothelial cell conditioned medium, was assayed through the Griess reaction…
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Medical study · Medical research · 1995
Chrysotile asbestos stimulates platelet-derived growth factor-AA production by rat lung fibroblasts in vitro: evidence for an autocrine loop
…However, message amplification using a reverse transcriptase polymerase chain reaction allowed detection of the B-chain message. A significant dose-dependent mitogenic effect of asbestos was found by using both a cell proliferation assay and nuclear…
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Medical study · Medical research · 1999
Production of reactive oxygen intermediates by human macrophages exposed to soot particles and asbestos fibers and increase in NF-kappa B p50/p105 mRNA
…100 micrograms/10(6) cells) compared with control experiments (p < 0.01). Identical exposure conditions for BM resulted in a similar reaction pattern (maximum 2.2-fold increase in ROI release; p < 0.05)…
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Medical study · Medical research · 1996
Lung cancer risk in relation to the CYP2E1 Rsa I genetic polymorphism among African-Americans and Caucasians in Los Angeles County
…Samples of white blood cell DNA sufficient for determination of the CYP2E1 Rsa I genotype by a polymerase chain reaction-based assay were obtained from 341 cases and 706 controls with data on lifetime smoking history…
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Medical study · Medical research · 1996
Comparative analysis of p16/CDKN2, p53 and ras gene alterations in human non-small cell lung cancers, with and without associated pulmonary asbestosis
…were examined for genetic abnormalities of p16/CDKN2, p53 and ras genes by single-strand conformation polymorphism analysis of polymerase chain reaction products (PCR-SSCP) and direct sequencing. Ten specimens were obtained from autopsies in which…
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Medical study · Medical research · 1997
Cellular mechanisms of reactive oxygen metabolite generation from human polymorphonuclear leukocytes induced by crocidolite asbestos
…an enhancement. We conclude that crocidolite-induced ROM generation involves a cell surface reaction due to influx of extracellular calcium through calcium channels and the activation of NADPH oxidase on the PMN cell membrane. This hypothesis…
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Medical study · Medical research · 1999
Possible role of ovarian epithelial inflammation in ovarian cancer
…risk to an excess number of lifetime ovulations or to elevations in steroid hormones. Inflammation may underlie ovulatory events because an inflammatory reaction is induced during the process of ovulation. Additional risk factors for ovarian cancer…
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Medical study · Medical research · 1990
Carcinosarcoma of the spermatic cord
…with PAS, Hale and mucicarmine stains, and showed positive reaction for immunohistochemical demonstration of both keratin and epithelial membrane antigen. The lumen content of the gland-like structures reacted positively for the carcinoembryonic antigen. Electron microscopy…
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Medical study · Medical research · 1996
The calcium binding protein calretinin is a selective marker for malignant pleural mesotheliomas of the epithelial type
…adenomatous or solid) and by the epithelial component of the mixed tumours. The immunohistochemical reaction is specific and reproducible. The tissues of the pulmonary parenchyma and of the pleura are negative for calretinin except for the…
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Medical study · Medical research · 1992
A novel biotinylated probe specific for hyaluronate. Its diagnostic value in diffuse malignant mesothelioma
…staining for hyaluronate. Furthermore, the staining reaction was classed as moderate or greater in 26 mesotheliomas (79%), thus suggesting the utility of this probe in the differential diagnosis of malignant mesothelioma versus adenocarcinoma. We conclude that…
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Medical study · Medical research · 1998
Cytokine gene therapy or infusion as treatment for solid human cancer
…excretion was detectable. At each cycle vaccinia-IL-2 mRNA (SQ [semi-quantitative] reverse transcription polymerase chain reaction) was maximal 24-72 h following injection reduced at 8 days and faded by 21 days. No tumor…
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Medical study · Medical research · 1998
Mineral dusts directly induce epithelial and interstitial fibrogenic mediators and matrix components in the airway wall
…titanium dioxide. Explants were then maintained in air organ culture, and the expression of genes encoding for various mediators and matrix components assessed by reverse transcriptase-polymerase chain reaction (RT-PCR). At 7 d, all dusts…
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Medical study · Medical research · 1999
Expression of genes coding for proteoglycans and Wilms' tumour susceptibility gene 1 (WT1) by variously differentiated benign human mesothelial cells
…to WT1, PGs and hyaluronan synthase, we studied their expression by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) analyses. The expressions of these genes were all associated with a variation in phenotypic differentiation. Cell lines…
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Medical study · Medical research · 1996
Renal cell carcinoma development in the rat independent of alterations at the VHL gene locus
…of this tumor suppressor gene in the development of these hereditary rat tumors. By using reverse transcriptase (RT)-polymerase chain reaction (PCR) analysis, the sequence of the rat VHL gene was determined over the portion of…
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Medical study · Medical research · 1994
Polyclonal human T-cell activation by silicate in vitro
…to silicate dust. T-cell receptor (TcR) V beta gene analysis on CD4- CD8- double-negative alpha beta T cells from scleroderma patients, using polymerase chain reaction (PCR), showed that certain V beta genes, V beta…
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Medical study · Medical research · 1999
Low dose cabergoline induced interstitial pneumonitis
…a "hypersensitivity-like" interstitial lung disease, which cleared almost completely within 2 months after simple drug withdrawal. Circumstantial evidence suggests a so far undescribed adverse lung reaction to cabergoline, devoid of the more usual pleural changes.
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Medical study · Medical research · 1990
Reactive lesions of the pleura. Immunohistochemical characterization
…The antibodies Leu M1, HEA 125 and BMA 130c (against CEA) showed no reaction in subserosal and mesothelial cells. With the antibody HMFG 2, however, a weak reaction could be observed. A distinction between reactive and…
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Medical study · Medical research · 1999
Kaposi's sarcoma following malignant mesothelioma
…of mesothelioma diagnosis and preceded the onset of Kaposi' sarcoma with a time lapse of 13 months. HHV8 DNA was detected by polymerase chain reaction in lesional Kaposi's sarcoma but not within mesothelioma. By immunostaining…
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Medical study · Medical research · 1998
Modulation of mitochondrial gene expression in pulmonary epithelial cells exposed to oxidants
…RNA was extracted, reverse transcribed, and amplified by polymerase chain reaction with degenerate primers to visualize alterations in gene expression. The seven clones obtained were sequenced and encoded the mitochondrial genes, NADH dehydrogenase subunits ND5 and…