226 results for “Reaction Department”, page 6 of 12
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Medical study · Medical research · 2013
Expression and role of GLUT-1, MCT-1, and MCT-4 in malignant pleural mesothelioma
…MPM (n = 35) and RMH (n = 20) specimens by immunohistochemistry and in frozen tissue, and MPM cell lines, by real-time reverse transcription-polymerase chain reaction and western blot analysis. GLUT-1, MCT-1…
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Medical study · Medical research · 2012
Extranodal marginal zone lymphoma of mucosa-associated lymphoid tissue type arising in the pleura with pleural fibrous plaques in a lathe worker
…pleura and diffuse infiltration of small- to medium-sized B lymphocytes. Polymerase chain reaction-based analysis detected monoclonal rearrangement of immunoglobulin heavy-chain genes. Histologic diagnosis was extranodal marginal zone lymphoma of mucosa-associated lymphoid tissue…
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Medical study · Medical research · 2011
miR-1254 and miR-574-5p: serum-based microRNA biomarkers for early-stage non-small cell lung cancer
…with early-stage NSCLC). Quantitative polymerase chain reaction (qPCR) was used to validate the profiling results in the discovery set and in a validation set of 31 controls and 22 patients with early-stage NSCLC. Additionally…
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Medical study · Medical research · 2013
RNA sequencing identifies fusion of the EWSR1 and YY1 genes in mesothelioma with t(14;22)(q32;q12)
…an EWSR1-YY1 fusion gene but not the reciprocal YY1-EWSR1 transcript. RT-polymerase chain reaction and sequencing showed that in both cases exon 8 of EWSR1 (nucleotide 1,139, accession number NM_013986 version 3…
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Medical study · Medical research · 2012
Iron overload signature in chrysotile-induced malignant mesothelioma
…closely associated with iron overload: repeated administration of an iron chelator, nitrilotriacetic acid, which promotes the Fenton reaction, significantly reduced the period required for carcinogenesis; massive iron deposition was found in the peritoneal organs with high…
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Medical study · Medical research · 2013
No association between simian virus 40 and diffuse malignant mesothelioma of the pleura in Iranian patients: a molecular and epidemiologic case-control study of 60 patients
…analysis. We related the patients with diffuse malignant mesothelioma to possible sites of exposure to asbestos. None of the DMMs nor any patient in the control group had SV40 genome on polymerase chain reaction (PCR). All…
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Medical study · Medical research · 2013
ZIC1 is silenced and has tumor suppressor function in malignant pleural mesothelioma
…cell growth and apoptosis, was investigated in MPM cell lines and tumors. ZIC1 expression and promoter methylation were evaluated in MPM cell lines and tumor samples by quantitative polymerase chain reaction (PCR), Combined Bisulfite Restriction Analysis…
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Medical study · Medical research · 2013
Reduced folate carrier and folylpolyglutamate synthetase, but not thymidylate synthase predict survival in pemetrexed-treated patients suffering from malignant pleural mesothelioma
…AICARFT, RFC, and FPGS levels in MPM and associations with clinical benefit from pemetrexed treatment. Samples from 63 patients were tested using immunohistochemistry (IHC) and quantitative polymerase chain reaction(qPCR) for expression levels of TS, GARFT…
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Medical study · Medical research · 2011
Expression and regulation of B7-H3 immunoregulatory receptor, in human mesothelial and mesothelioma cells: immunotherapeutic implications
…agents in modulating levels of B7-H3 expression in MM cells was also studied. Reverse transcriptase-polymerase chain reaction (RT-PCR) demonstrated that B7-H3 mRNA was consistently detectable in mesothelial and MM cells investigated; however…
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Medical study · Medical research · 2013
Differential expression of extracellular matrix constituents and cell adhesion molecules between malignant pleural mesothelioma and mesothelial hyperplasia
…carcinogenesis. We analyzed a panel of 84 genes involved in extracellular matrix remodeling and cell adhesion by polymerase chain reaction (PCR) array in 15 samples of epithelioid mesothelioma and 10 samples of reactive mesothelial hyperplasia (MH…
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Medical study · Medical research · 2014
ERC/mesothelin is expressed in human gastric cancer tissues and cell lines
…MKN-7, MKN-74, NUGC-3, NUGC-4 and TMK-1) using reverse transcription-polymerase chain reaction, flow cytometry, immunohistochemistry and immunoblotting. We also examined the N-ERC/mesothelin concentrations in the supernatants of cultured cells…
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Medical study · Medical research · 2013
Overexpression of EPH receptor B2 in malignant mesothelioma correlates with oncogenic behavior
…we investigated EPHB2 in MM tumors compared with normal mesothelium. EPHB2 and downstream target expression were evaluated using reverse-transcriptase polymerase chain reaction and immunoblotting methods. The biological significance of EPHB2 in MM was evaluated using…
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Medical study · Medical research · 2014
Morphological changes during enhanced carbonation of asbestos containing material and its comparison to magnesium silicate minerals
…mineral carbonation. The study revealed that oxalate even at a low concentration of 0.1M was effective in enhancing the extent of ACM carbonation and higher reaction temperatures also resulted in increased ACM carbonation. Formation of…
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Medical study · Medical research · 2011
A₃ receptors are overexpressed in pleura from patients with mesothelioma and reduce cell growth via Akt/nuclear factor-κB pathway
…between adenosine and MM, the presence and functionality of ARs were explored in human healthy mesothelial cells (HMC) and in malignant mesothelioma cells (MMC). ARs were analyzed by using reverse transcriptase-polymerase chain reaction, Western blotting…
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Medical study · Medical research · 2015
Poorly differentiated neoplasms of unknown primary site: diagnostic usefulness of a molecular cancer classifier assay
…PDNs without a definitive lineage diagnosed by histology or IHC (median 18 stains, range 9-46). Biopsies from these 30 patients had MCCA (92-gene reverse transcriptase-polymerase chain reaction mRNA) performed. Additional IHC, gene sequencing…
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Medical study · Medical research · 2010
No implication of Simian virus 40 in pathogenesis of malignant pleural mesothelioma in Slovenia
…antibody against Simian virus 40 (Pab280). SV40-expressing Wi-38 cells were used as positive control in both PCR and immunohistochemistry. In real-time polymerase chain reaction analyses, only 4 samples gave products with primer pairs…
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Medical study · Medical research · 2014
Tumors that mimic asbestos-related mesothelioma: time to consider a genetics-based tumor registry?
…Some of these metastatic malignancies, particularly carcinomas and sarcomas of the pleura, pericardium and peritoneum, may undergo desmoplastic reaction in the pleura, thereby mimicking mesothelioma, rather than the primary tumor. Encasement of the lung by direct…
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Medical study · Medical research · 2011
MicroRNAs dysregulation in human malignant pleural mesothelioma
…with microarray approach. These results were confirmed by real-time quantitative reverse-transcriptase polymerase chain reaction and Western blotting. A comparative analysis of miRNA expression in MPM and HMCs was carried out. Microarray profiling showed different…
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Medical study · Medical research · 2014
Epidermal growth factor receptor mutations in 510 Finnish non--small-cell lung cancer patients
…embedded, formalin-fixed tumor material using the following real-time polymerase chain reaction-based kits: Therascreen EGFR PCR Kit and cobas EGFR Mutation Test. EGFR mutation frequency was 11.4% and all positive cases were adenocarcinomas…
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Medical study · Medical research · 2014
Well-differentiated papillary mesothelioma of the tunica vaginalis: a case study and review of the literature
…structures with focal coagulative necrosis were present. In addition to immunohistochemistry, simian virus 40 DNA was also tested by polymerase chain reaction. This report presents one case of this rare entity, its clinical and macroscopic features…