8 results for “Reaction Department”
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Medical study · Medical research · 1991
Human mononuclear phagocytes express the insulin-like growth factor-II/mannose-6-phosphate receptor
…AM lavaged from individuals with asbestos exposure or from normal volunteers. Using the polymerase chain reaction and reverse transcriptase to reverse-transcribe mRNA from mononuclear phagocytes, specific IGF-II/M6P receptor cDNA was amplified and detected…
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Medical study · Medical research · 2002
The presence of simian-virus 40 sequences in mesothelioma and mesothelial cells is associated with high levels of vascular endothelial growth factor
…detected in conditioned media of each of two MM cells that tested positive for SV40 by PCR amplification and Southern blot hybridization and for Tag transcript by reverse transcription- polymerase chain reaction (RT-PCR) and immunoprecipitation…
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Medical study · Medical research · 1997
Absence of p53 mutations in malignant mesotheliomas
…mesotheliomas. Thirteen malignant mesotheliomas collected over the preceding 12 yr and maintained in paraffin blocks were examined by polymerase chain reaction (PCR) followed by both single-strand conformation polymorphism (SSCP) and denaturing gradient-gel electrophoresis (DGGE)…
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Medical study · Medical research · 1998
Asbestos fibers and interleukin-1 upregulate the formation of reactive nitrogen species in rat pleural mesothelial cells
…polymerase chain reaction (RT-PCR), increased progressively from 2 to 12 h in IL-1beta-containing cultures. Nitrite (NO2-), the stable oxidation product of .NO in mesothelial cell conditioned medium, was assayed through the Griess reaction…
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Medical study · Medical research · 1995
Chrysotile asbestos stimulates platelet-derived growth factor-AA production by rat lung fibroblasts in vitro: evidence for an autocrine loop
…However, message amplification using a reverse transcriptase polymerase chain reaction allowed detection of the B-chain message. A significant dose-dependent mitogenic effect of asbestos was found by using both a cell proliferation assay and nuclear…
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Medical study · Medical research · 1994
Asbestos fibers and interferon-gamma up-regulate nitric oxide production in rat alveolar macrophages
…in macrophage conditioned medium, was assayed by the Griess reaction. Production of NO2- by AM was significantly increased by both chrysotile (P < 0.01) and crocidolite (P < 0.05) asbestos fibers (10 micrograms/ml…
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Medical study · Medical research · 2000
Serologic responses in patients with malignant mesothelioma: evidence for both public and private specificities
…protein. Antibodies to topoisomerase II beta were found in 13 of 14 patients and in 6 of 16 volunteers, though the volunteers' reactions were generally weak. Antibodies to U2AF(65) were found in two patients. Each…
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Medical study · Medical research · 1995
Iron and reactive oxygen species in the asbestos-induced tumor necrosis factor-alpha response from alveolar macrophages
…and for interleukin-1 alpha about 10-fold. The authors concluded that free radicals produced by iron-catalyzed reactions mediate asbestos-induced TNF-alpha gene expression and secretion. They noted that deferoxamine may not remove all…