21 results for “Reaction Department”, page 1 of 2
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Medical study · Medical research · 2013
Phase 2 study of sorafenib in malignant mesothelioma previously treated with platinum-containing chemotherapy
…at 8 weeks. The most common severe side effects were fatigue (15%), a hand-foot skin reaction called palmar-plantar erythrodysesthesia (13%) and rash (9%). Eleven patients (21%) stopped treatment because of side effects. PET scan…
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Medical study · Medical research · 2011
Aromatase inhibitor exemestane has antiproliferative effects on human mesothelioma cells
…aromatase (CYP19A1) in malignant mesothelioma (MM). We found CYP19A1 in five human MM cell lines using reverse transcription polymerase chain reaction and Western immunoblots and in a group of samples from patients with MM by immunohistochemistry…
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Medical study · Medical research · 2016
Functional Analysis of the Adrenomedullin Pathway in Malignant Pleural Mesothelioma
…protein 2 (CLR/RAMP2) and calcitonin receptor-like receptor/receptor activity-modifying protein 3 (CLR/RAMP3). Real time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) was used to assess the steady-state levels of AM…
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Medical study · Medical research · 2021
UHRF1 Is a Novel Druggable Epigenetic Target in Malignant Pleural Mesothelioma
…in malignant pleural mesothelioma (MPM). This study was undertaken to explore the therapeutic potential of targeting UHRF1 in MPM. Microarray, real-time quantitative reverse transcription-polymerase chain reaction, immunoblot, and immunohistochemistry techniques were used to evaluate…
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Medical study · Medical research · 2017
A Novel BRCA1-Associated Protein-1 Isoform Affects Response of Mesothelioma Cells to Drugs Impairing BRCA1-Mediated DNA Repair
…BAP1 and investigate the effects of its expression on drug sensitivity in MPM. Expression of BAP1 isoforms was detected by quantitative polymerase chain reaction in MPM and normal mesothelium cell lines and tumor and nontumor samples…
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Medical study · Medical research · 2018
Dysregulated Expression of the MicroRNA miR-137 and Its Target YBX1 Contribute to the Invasive Characteristics of Malignant Pleural Mesothelioma
…methylation, and copy number status of miR-137 and its host gene MIR137HG were assessed by polymerase chain reaction. Luciferase reporter assays confirmed a direct interaction between miR-137 and Y-box binding protein 1 gene…
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Medical study · Medical research · 2017
Tumor Suppressor microRNAs Contribute to the Regulation of PD-L1 Expression in Malignant Pleural Mesothelioma
…from samples from patients undergoing an operation (pleurectomy with or without decortication). MicroRNA expression was analyzed by reverse-transcriptase quantitative polymerase chain reaction. Regulation of PD-L1 expression in cell lines was assessed after transfection with…
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Medical study · Medical research · 2012
Activated leukocyte cell-adhesion molecule (ALCAM) promotes malignant phenotypes of malignant mesothelioma
…MM cell lines. Activated leukocyte cell-adhesion molecule (ALCAM) expression in MM cell lines was analyzed with quantitative reverse transcription-polymerase chain reaction and Western blot analyses in 47 primary MM specimens with immunohistochemistry. ALCAM knockdown…
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Medical study · Medical research · 2009
Survey and biological insights of pemetrexed-related therapeutic improvement in mesothelioma: The Nancy Centre of Biological Resources' Mesothelioma Cohort
…France (1989-2007). Blood or paired tumoral and normal samples were collected from the last 210 French patients to study the TCII single nucleotide polymorphisms at the codon 259 (quantitative polymerase chain reaction). Results were compared…
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Medical study · Medical research · 2011
miR-1254 and miR-574-5p: serum-based microRNA biomarkers for early-stage non-small cell lung cancer
…with early-stage NSCLC). Quantitative polymerase chain reaction (qPCR) was used to validate the profiling results in the discovery set and in a validation set of 31 controls and 22 patients with early-stage NSCLC. Additionally…
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Medical study · Medical research · 2013
ZIC1 is silenced and has tumor suppressor function in malignant pleural mesothelioma
…cell growth and apoptosis, was investigated in MPM cell lines and tumors. ZIC1 expression and promoter methylation were evaluated in MPM cell lines and tumor samples by quantitative polymerase chain reaction (PCR), Combined Bisulfite Restriction Analysis…
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Medical study · Medical research · 2013
Reduced folate carrier and folylpolyglutamate synthetase, but not thymidylate synthase predict survival in pemetrexed-treated patients suffering from malignant pleural mesothelioma
…AICARFT, RFC, and FPGS levels in MPM and associations with clinical benefit from pemetrexed treatment. Samples from 63 patients were tested using immunohistochemistry (IHC) and quantitative polymerase chain reaction(qPCR) for expression levels of TS, GARFT…
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Medical study · Medical research · 2013
Differential expression of extracellular matrix constituents and cell adhesion molecules between malignant pleural mesothelioma and mesothelial hyperplasia
…carcinogenesis. We analyzed a panel of 84 genes involved in extracellular matrix remodeling and cell adhesion by polymerase chain reaction (PCR) array in 15 samples of epithelioid mesothelioma and 10 samples of reactive mesothelial hyperplasia (MH…
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Medical study · Medical research · 2013
Overexpression of EPH receptor B2 in malignant mesothelioma correlates with oncogenic behavior
…we investigated EPHB2 in MM tumors compared with normal mesothelium. EPHB2 and downstream target expression were evaluated using reverse-transcriptase polymerase chain reaction and immunoblotting methods. The biological significance of EPHB2 in MM was evaluated using…
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Medical study · Medical research · 2011
MicroRNAs dysregulation in human malignant pleural mesothelioma
…with microarray approach. These results were confirmed by real-time quantitative reverse-transcriptase polymerase chain reaction and Western blotting. A comparative analysis of miRNA expression in MPM and HMCs was carried out. Microarray profiling showed different…
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Medical study · Medical research · 2014
Epidermal growth factor receptor mutations in 510 Finnish non--small-cell lung cancer patients
…embedded, formalin-fixed tumor material using the following real-time polymerase chain reaction-based kits: Therascreen EGFR PCR Kit and cobas EGFR Mutation Test. EGFR mutation frequency was 11.4% and all positive cases were adenocarcinomas…
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Medical study · Medical research · 2012
Increased circulating miR-625-3p: a potential biomarker for patients with malignant pleural mesothelioma
…healthy controls. miRNAs with significantly different abundance between cases and controls were validated in a larger series of MM patients and in an independent series of MM patients using quantitative real-time polymerase chain reaction. Levels…
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Medical study · Medical research · 2014
Frequent coamplification and cooperation between C-MYC and PVT1 oncogenes promote malignant pleural mesothelioma
…lines. Copy number analysis was measured by quantitative polymerase chain reaction and fluorescence in situ hybridization. Copy number analysis revealed copy number gains (CNGs) in chromosomal region 8q24 in six of 12 MPM cell lines. MicroRNA…
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Medical study · Medical research · 2019
Proton Beam Therapy and Immune Checkpoint Inhibitors in Malignant Pleural Mesothelioma
…and a biopsy showed alveolar injury. Corticosteroids led to rapid symptom resolution. The authors hypothesized that proton therapy caused immune "priming" and a later "recall" reaction. They concluded that the combination, particularly with very large treatment…
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Medical study · Medical research · 2018
New Insights on Diagnostic Reproducibility of Biphasic Mesotheliomas: A Multi-Institutional Evaluation by the International Mesothelioma Panel From the MESOPATH Reference Center
…the usefulness of FISH detection of CDKN2A(p16) HD compared to BAP1 loss on the spindle cell component for the separation in ambiguous cases between benign florid stromal reaction from true sarcomatoid component of biphasic mesothelioma…