558 results for “Reaction Department”, page 12 of 28
-
Medical study · Medical research · 2016
Effects of Chrysotile Exposure in Human Bronchial Epithelial Cells: Insights into the Pathogenic Mechanisms of Asbestos-Related Diseases
…glycogen synthase kinase-3β (GSK-3β), and Akt were performed to confirm their involvement in asbestos-induced EMT. Real-time polymerase chain reaction (PCR), Western blotting, and gelatin zymography were performed to detect mRNA…
-
Medical study · Medical research · 2024
Small Extracellular Vesicle-Derived Circular RNA hsa_circ_0007386 as a Biomarker for the Diagnosis of Pleural Mesothelioma
…in cancer. This study aimed to identify potential biomarkers for PM diagnosis by investigating the expression of specific circRNA gene pattern (hsa_circ_0007386) in cells and sEVs using digital polymerase chain reaction (dPCR). For this…
-
Medical study · Medical research · 2021
Programmed cell death 1 ligand 1 (PD-L1) expression is associated with poor prognosis of malignant pleural mesothelioma patients with good performance status
…expression in MPM prognosis. Biopsy samples from 198 patients diagnosed with MPM were examined by immunohistochemistry (IHC) and reverse transcription-polymerase chain reaction (RT-PCR) to evaluate PD-L1 protein and gene expression. For PD-L1…
-
Medical study · Medical research · 2017
Silicosis and autoimmunity
…Although the importance of the NACHT, LRR and PYD domains-containing protein 3 (NALP3) inflammasome as the initial immune reaction against silica particles has been identified, the mechanisms involved that lead to various autoimmune diseases in…
-
Medical study · Medical research · 2018
A clean and membrane-free chlor-alkali process with decoupled Cl2 and H2/NaOH production
…reaction (H2O → H2) and an anodic Na+ de-intercalation reaction (Na0.44MnO2 → Na0.44-xMnO2), during which NaOH is produced in the electrolyte solution. The second step depends on a cathodic Na+ intercalation reaction…
-
Medical study · Medical research · 2016
Dual preventive benefits of iron elimination by desferal in asbestos-induced mesothelial carcinogenesis
…its effects on asbestos-induced peritoneal inflammation and iron deposition, as well as oxidative stress. Nitrilotriacetate was used to promote an iron-catalyzed Fenton reaction as a positive control. Desferal significantly decreased peritoneal fibrosis, iron deposition…
-
Medical study · Medical research · 2020
Medical Challenge Posed by Retroperitoneal Fibrosis: Case Reports and Literature Review
…tomography (CT) and magnetic resonance imaging (MRI) are the most reliable imaging modalities. The goal of treatment is to stop the progression of the fibroinflammatory reaction. The first line of treatment is usually with medical management…
-
Medical study · Medical research · 2019
The ROMP: A Powerful Approach to Synthesize Novel pH-Sensitive Nanoparticles for Tumor Therapy
…molecule can be grafted by click chemistry. A cleavage reaction of the chemical bond between NPs and drugs through the contact of NPs with a medium presenting an acidic pH, which is typically a cancer tumor…
-
Medical study · Medical research · 2017
Identification of DAB2 and Intelectin-1 as Novel Positive Immunohistochemical Markers of Epithelioid Mesothelioma by Transcriptome Microarray Analysis for Its Differentiation From Pulmonary Adenocarcinoma
…adenocarcinoma. The increased mRNA expression of DAB2 and Intelectin-1 was validated by reverse transcriptase polymerase chain reaction of RNA from tumor tissue and protein expression was validated by Western blotting of 5 mesothelioma cell lines…
-
Medical study · Medical research · 2020
Induction of Gamma-Glutamyltransferase Activity and Consequent Pro-oxidant Reactions in Human Macrophages Exposed to Crocidolite Asbestos
…its fibers as well as to its content of iron. The latter can in fact react with macrophage-derived hydrogen peroxide in the so called Fenton reaction, giving rise to highly reactive and mutagenic hydroxyl radical…
-
Medical study · Medical research · 2016
Kinesin family members KIF11 and KIF23 as potential therapeutic targets in malignant pleural mesothelioma
…and new molecular targets for treatments are urgently needed. Using quantitative reverse transcription-polymerase chain reaction (RT-PCR) and an RNA interference-based screening, we screened two kinesin family members as potential therapeutic targets for MPM…
-
Medical study · Medical research · 2020
The role of endothelial nitric oxide synthase gene polymorphisms in patients with lung cancer
…Designated polymorphisms were identified by polymerase chain reaction (PCR) and/or restriction fragment length polymorphism (RFLP). Our study showed that the frequencies of the b/b genotype and b allele of eNOS gene intron 4 VNTR…
-
Medical study · Medical research · 1998
SV40-like DNA sequences in pleural mesothelioma, bronchopulmonary carcinoma, and non-malignant pulmonary diseases
…tumours, and 33 non-malignant samples) and 38 additional samples distant from tumours were studied for the occurrence of SV40-like DNA sequences by polymerase chain reaction (PCR) amplification followed by hybridization with specific probes. Sequences…
-
Medical study · Medical research · 2000
Simian virus (SV) 40 like sequences in cell lines and tumour biopsies from Australian malignant mesotheliomas
…its gene products in tissue derived from mesotheliomas would detract from this possibility. We used polymerase chain reaction from three pairs of primers to amplify different regions of the large T antigen from DNA from cell…
-
Medical study · Medical research · 1999
VEGF and VEGF type C play an important role in angiogenesis and lymphangiogenesis in human malignant mesothelioma tumours
…cell lines, 54 MPM tumours and five normal pleural tissues. Expression levels for receptors and ligands were assessed by semiquantitative reverse transcriptase polymerase chain reaction analysis. Microvessels were highlighted by immunohistochemical staining for factor VIII. The…
-
Medical study · Medical research · 2010
Fluorescence in situ hybridization in the definitive diagnosis of malignant mesothelioma in effusion cytology
…In case of a negative result, the corresponding MM biopsy specimen was analyzed. Methylation-specific polymerase chain reaction (MSP) for p16, p14, and p15 was performed on FISH-negative MM biopsy specimens. Seventy-nine percent of…
-
Medical study · Medical research · 1978
Experimental asbestosis: an investigation of functional and pathological disturbances. II. Results for chrysotile and amosite exposures
…not until about 70 weeks for animals exposed when three months old. No fibrotic reaction was seen during the 70 weeks of the amosite experiments. The relationships between pathological and functional changes throughout these experiments are…
-
Medical study · Medical research · 1998
Pathogenesis of experimentally induced asbestos mesothelioma in rats
…and small cells covering spaces over the ribs. Inflammatory reaction and permanent pathological regenerative processes were observed in the mesothelium during 24 months after inoculation of asbestos fibres. Different lesions which we regarded as preneoplastic or…
-
Medical study · Medical research · 1983
Diseases associated with asbestos exposure. Diagnostic perspectives in forensic pathology
…and its numerous applications. Its biological effects, which include fibrogenesis and carcinogenesis, may be related to the cellular reaction, to its large specific surface, or to the size and shape of the fibers. Its oncogenic action…
-
Medical study · Medical research · 1991
Human mononuclear phagocytes express the insulin-like growth factor-II/mannose-6-phosphate receptor
…AM lavaged from individuals with asbestos exposure or from normal volunteers. Using the polymerase chain reaction and reverse transcriptase to reverse-transcribe mRNA from mononuclear phagocytes, specific IGF-II/M6P receptor cDNA was amplified and detected…