558 results for “Reaction Department”, page 15 of 28
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Medical study · Medical research · 2013
ZIC1 is silenced and has tumor suppressor function in malignant pleural mesothelioma
…cell growth and apoptosis, was investigated in MPM cell lines and tumors. ZIC1 expression and promoter methylation were evaluated in MPM cell lines and tumor samples by quantitative polymerase chain reaction (PCR), Combined Bisulfite Restriction Analysis…
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Medical study · Medical research · 2007
Asbestos-induced peribronchiolar cell proliferation and cytokine production are attenuated in lungs of protein kinase C-delta knockout mice
…decreased in PKCdelta-/- mice. At 9 days, microarray and quantitative reverse transcriptase-polymerase chain reaction analysis of lung tissues revealed increased mRNA levels of the profibrotic cytokine, IL-4, in asbestos-exposed wild-type mice but…
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Medical study · Medical research · 1997
Cellular mechanisms of reactive oxygen metabolite generation from human polymorphonuclear leukocytes induced by crocidolite asbestos
…an enhancement. We conclude that crocidolite-induced ROM generation involves a cell surface reaction due to influx of extracellular calcium through calcium channels and the activation of NADPH oxidase on the PMN cell membrane. This hypothesis…
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Medical study · Medical research · 2000
'Mucin-positive' epithelial mesothelioma of the peritoneum: an unusual diagnostic pitfall
…crystals. Repeat histochemical analysis following hyaluronidase pretreatment revealed a significantly diminished diastase-resistant periodic acid-Schiff reaction. This case of primary peritoneal 'mucin-positive' epithelial mesothelioma demonstrates morphological and histochemical mimicry with diffuse gastric adenocarcinoma. A…
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Medical study · Medical research · 1981
Diminished suppressor cell function in patients with asbestosis
…patients. Skin reaction to dinitrochlorobenzene (DNCB) was not depressed, although lymphocytes of patients giving positive skin test reactions demonstrated a significantly lower (P less than 0.001) proliferative response to dinitrobenzene sulphonic acid (DNBSO3) in vitro…
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Medical study · Medical research · 1999
Possible role of ovarian epithelial inflammation in ovarian cancer
…risk to an excess number of lifetime ovulations or to elevations in steroid hormones. Inflammation may underlie ovulatory events because an inflammatory reaction is induced during the process of ovulation. Additional risk factors for ovarian cancer…
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Medical study · Medical research · 1990
Carcinosarcoma of the spermatic cord
…with PAS, Hale and mucicarmine stains, and showed positive reaction for immunohistochemical demonstration of both keratin and epithelial membrane antigen. The lumen content of the gland-like structures reacted positively for the carcinoembryonic antigen. Electron microscopy…
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Medical study · Medical research · 2013
Reduced folate carrier and folylpolyglutamate synthetase, but not thymidylate synthase predict survival in pemetrexed-treated patients suffering from malignant pleural mesothelioma
…AICARFT, RFC, and FPGS levels in MPM and associations with clinical benefit from pemetrexed treatment. Samples from 63 patients were tested using immunohistochemistry (IHC) and quantitative polymerase chain reaction(qPCR) for expression levels of TS, GARFT…
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Medical study · Medical research · 1986
Studies on surface properties of asbestos. II. Role of dimensional characteristics and surface properties of mineral fibers in the induction of pleural tumors
…mineral and therefore the nature of active surface sites, and the aspect ratio of the particle. These two parameters can intervene in a mechanism of model reaction involving the basic properties of lateral faces of fibers.
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Medical study · Medical research · 2002
Aberrant methylation of the CDKN2a/p16INK4a gene promoter region in preinvasive bronchial lesions: a prospective study in high-risk patients without invasive cancer
…the CDKN2A gene. Methylation-specific polymerase chain reaction was performed on DNA extracted from pure bronchial cell populations derived from biopsies and detection of p16 protein was studied by immunohistochemistry on contiguous parallel biopsies. Aberrant methylation…
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Medical study · Medical research · 1996
The calcium binding protein calretinin is a selective marker for malignant pleural mesotheliomas of the epithelial type
…adenomatous or solid) and by the epithelial component of the mixed tumours. The immunohistochemical reaction is specific and reproducible. The tissues of the pulmonary parenchyma and of the pleura are negative for calretinin except for the…
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Medical study · Medical research · 1992
A novel biotinylated probe specific for hyaluronate. Its diagnostic value in diffuse malignant mesothelioma
…staining for hyaluronate. Furthermore, the staining reaction was classed as moderate or greater in 26 mesotheliomas (79%), thus suggesting the utility of this probe in the differential diagnosis of malignant mesothelioma versus adenocarcinoma. We conclude that…
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Medical study · Medical research · 1998
Cytokine gene therapy or infusion as treatment for solid human cancer
…excretion was detectable. At each cycle vaccinia-IL-2 mRNA (SQ [semi-quantitative] reverse transcription polymerase chain reaction) was maximal 24-72 h following injection reduced at 8 days and faded by 21 days. No tumor…
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Medical study · Medical research · 2011
Expression and regulation of B7-H3 immunoregulatory receptor, in human mesothelial and mesothelioma cells: immunotherapeutic implications
…agents in modulating levels of B7-H3 expression in MM cells was also studied. Reverse transcriptase-polymerase chain reaction (RT-PCR) demonstrated that B7-H3 mRNA was consistently detectable in mesothelial and MM cells investigated; however…
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Medical study · Medical research · 2005
Identification of novel candidate oncogenes and tumor suppressors in malignant pleural mesothelioma using large-scale transcriptional profiling
…normal and tumor tissues, and tumors with different morphologies. Microarray gene expression data were confirmed using quantitative reverse transcriptase-polymerase chain reaction and protein analysis for three novel candidate oncogenes (NME2, CRI1, and PDGFC) and one…
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Medical study · Medical research · 1983
Method for removing the ferruginous coating from asbestos bodies
…treatment consisted of an 8% oxalic acid bath used at various temperatures. It was determined that at 75 degrees C the reaction resulted in removal of the ferruginous coat, leaving an exposed core for further analysis…
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Medical study · Medical research · 1998
Mineral dusts directly induce epithelial and interstitial fibrogenic mediators and matrix components in the airway wall
…titanium dioxide. Explants were then maintained in air organ culture, and the expression of genes encoding for various mediators and matrix components assessed by reverse transcriptase-polymerase chain reaction (RT-PCR). At 7 d, all dusts…
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Medical study · Medical research · 2007
Beyond foreign-body-induced carcinogenesis: impact of reactive oxygen species derived from inflammatory cells in tumorigenic conversion and tumor progression
…of foreign-body-induced tumorigenesis. What is notable is that the foreign bodies themselves are unrelated to each other, whereas commonly underlying in them is to induce inflammatory reaction, especially stromal proliferation, where those exogenous materials…
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Medical study · Medical research · 2001
Changes in DNA 8-hydroxyguanine levels, 8-hydroxyguanine repair activity, and hOGG1 and hMTH1 mRNA expression in human lung alveolar epithelial cells induced by crocidolite asbestos
…and human MUtT homologue (hMTH1). This was done using a high-performance liquid chromatography system equipped with an electrochemical detector, endonuclease nicking assay and reverse transcription polymerase chain reaction, respectively. Crocidolite induced the formation of 8…
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Medical study · Medical research · 2001
Glypican-3 expression in Wilms tumor and hepatoblastoma
…and protein were extracted from Wilms tumor and hepatoblastoma tissue samples and GPC3 levels were measured in these extracts by Northern blotting, reverse transcription polymerase chain reaction, and immunoblotting. In contrast to published results with carcinomas…