1,074 results for “Reaction Department”, page 19 of 54
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Lexicon
Tyrosine Nitration
…oxidative stress. It serves as a measurable marker of the chronic tissue injury seen in asbestos-exposed lungs and pleura. Origin The reaction was characterized in the early 1990s by Joseph Beckman and colleagues, who showed…
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Medical study · Medical research · 2021
MicroRNA expression in lung tissues of asbestos-exposed mice: Upregulation of miR-21 and downregulation of tumor suppressor genes Pdcd4 and Reck
…mice four times weekly. We extracted total RNA from lung tissues and performed microarray analysis for miRNA and gene expression. We also carried out real-time polymerase chain reaction (PCR), Western blotting, and immunohistochemistry to confirm…
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Medical study · Medical research · 2016
Functional Analysis of the Adrenomedullin Pathway in Malignant Pleural Mesothelioma
…protein 2 (CLR/RAMP2) and calcitonin receptor-like receptor/receptor activity-modifying protein 3 (CLR/RAMP3). Real time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) was used to assess the steady-state levels of AM…
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Medical study · Medical research · 2021
Osteopontin mRNA expression by rat mesothelial cells exposed to multi-walled carbon nanotubes as a potential biomarker of chronic neoplastic transformation in vitro
…were used as a positive control. Osteopontin (OPN) mRNA was assayed as a biomarker of transformation by real time quantitative polymerase chain reaction (qPCR) and transformation was determined by a cell invasion assay. Exposure to rMWCNTs…
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Medical study · Medical research · 2016
Young investigator challenge: MicroRNA-21/MicroRNA-126 profiling as a novel tool for the diagnosis of malignant mesothelioma in pleural effusion cytology
…authors evaluated the utility of an miRNA-based classifier as a complement to cytology. Quantitative reverse transcriptase-polymerase chain reaction analysis of 15 miRNAs was performed in mesothelial (MET-5A) and MM (H28 and H2052) cell…
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Medical study · Medical research · 2016
Low homozygous/high heterozygous deletion status by p16 FISH correlates with a better prognostic group than high homozygous deletion status in malignant pleural mesothelioma
…FISH was performed in 93 MPMs and 47 RMHs. Real-time polymerase chain reaction (PCR) and methylation specific PCR (MSP) were also performed for cases in which DNA was available. Overall survival (OS) was assessed via…
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Medical study · Medical research · 2020
Targeting ROR1 in combination with pemetrexed in malignant mesothelioma cells
…Microarray, quantitative polymerase chain reaction, and Western blot analyses showed that there were differences in the suppression of mRNA and proteins between H2452 and H28 cells transfected with siROR1 compared with those transfected with control siRNA…
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Medical study · Medical research · 2021
UHRF1 Is a Novel Druggable Epigenetic Target in Malignant Pleural Mesothelioma
…in malignant pleural mesothelioma (MPM). This study was undertaken to explore the therapeutic potential of targeting UHRF1 in MPM. Microarray, real-time quantitative reverse transcription-polymerase chain reaction, immunoblot, and immunohistochemistry techniques were used to evaluate…
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Article · JM Knowledge Chronology · 1955
G. W. H. Schepers, T. M. Durkan and A. B. Delahant, "Effect of Inhaled Commercial Hydrous Calcium Silicate Dust on Animal Tissues" (1955)
…describing the finding as a "mild asbestosis reaction" (Hazard to Brought). In Johns-Manville's chronology Johns-Manville's own chronology of what the company knew describes this document as follows: Schepers published article on 7…
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Medical study · Medical research · 2019
Clinical significance of serum DRAM1 mRNA, ARSA mRNA, hsa-miR-2053 and lncRNA-RP1-86D1.3 axis expression in malignant pleural mesothelioma
…miR-2053) and long noncoding RNA ( lncRNA-RP1-86D1.3). Then, quantitative real-time polymerase chain reaction (qPCR) validation in sera of 60 MPM patients, 20 chronic asbestos exposure patients, and 20 healthy volunteers was done…
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Medical study · Medical research · 2019
Modeling Epidermal Growth Factor Inhibitor-mediated Enhancement of Photodynamic Therapy Efficacy Using 3D Mesothelioma Cell Culture
…EGFR expression associating with worse outcomes after Photofrin-mediated PDT for MPM, and the extensive desmoplastic reaction associated with MPM influences tumor phenotype and therapeutic response. Since extracellular matrix (ECM) proteins accrued during stroma development can…
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Medical study · Medical research · 2020
LOX family and ZFPM2 as novel diagnostic biomarkers for malignant pleural mesothelioma
…that could act as MPM biomarkers. We carried out additional molecular analyses of these potential markers using MPM patient tissue samples via quantitative polymerase chain reaction. We identified Lysyl oxidase (LOX), Lysyl oxidase homologs 1&2…
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Medical study · Medical research · 2024
Telomere length and TERT polymorphisms as biomarkers in asbestos-related diseases
…length was also determined in samples from MM patients before and after chemotherapy. Telomere length was determined by monochromatic multiplex quantitative polymerase chain reaction (PCR), while competitive allele-specific PCR was used to genotype hTERT rs10069690…
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Medical study · Medical research · 2018
BAP1 gene mutations in Egyptian patients with advanced sporadic malignant pleural mesothelioma (MPM): relation with clinical outcomes and survival
…and treated as advanced MPM. BAP1 gene mutations were assessed from circulating tumor cells (CTCs) by polymerase chain reaction (PCR) and sequencing and these mutations have been confirmed using the tumor tissue. BAP1 immunohistochemistry was performed…
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Medical study · Medical research · 2017
In response to Sharing different perspectives to understand asbestos-induced carcinogenesis: A comment to Jiang et al. (2016) by Alessandro Francesco Gualtieri (2017)
…hemoglobin. They stated that catalytic Fe(II) is more important than Fe(III) because it initiates a Fenton reaction. They described two roles of macrophages: scavenging fibers and promoting inflammation. They stated that chrysotile is carcinogenic…
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Medical study · Medical research · 2018
In vitro genotoxicity of asbestos substitutes induced by coupled stimulation of dissolved high-valence ions and oxide radicals
…acellular ·OH which formed at the fiber surface at sites with loose/unsaturated bonds, as well as by catalytic reaction through dissolved iron. Accumulated mechanical and radical stimulation induced the intracellular reactive oxygen species (ROS) elevation…
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Medical study · Medical research · 2015
DNA copy number gains in malignant pleural mesothelioma
…The molecular pathogenesis of MPM remains unclear. The present study analyzed the frequency of various genomic copy number gains (CNGs) in MPM using reverse transcription-quantitative polymerase chain reaction. A total of 83 primary MPMs and…
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Medical study · Medical research · 2017
Magnesium oxide production from chrysotile asbestos detoxification with oxalic acid treatment
…treatments destructed the Chr structure while a new biomaterial, Glushinskite (Gls) was formed by the reaction between Oxac with the outer Brucite (Brc) (MgO2) layer surface of Chr. Oxac 0.05M was selected as the optimal…
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Medical study · Medical research · 2021
Re-evaluating the pathogenicity of the c.783+2T>C BAP1 germline variant
…None of the three available tumors showed an allelic loss, there was no discernable effect on alternative splicing based on reverse-transcription polymerase chain reaction, and there was no decrease or loss of somatic protein expression…
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Medical study · Medical research · 2020
Attenuation of oxidative stress and chromosomal aberrations in cultured macrophages and pulmonary cells following self-sustained high temperature synthesis of asbestos
…However, there was an attenuation of redox status imbalance and an absence of chromosomal aberrations, which probably reflects the loss of the asbestos fibrous structure following SHS reaction, as demonstrated by electron microscopy analyses. In conclusions…