1,074 results for “Reaction Department”, page 22 of 54
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Medical study · Medical research · 1991
Human mononuclear phagocytes express the insulin-like growth factor-II/mannose-6-phosphate receptor
…AM lavaged from individuals with asbestos exposure or from normal volunteers. Using the polymerase chain reaction and reverse transcriptase to reverse-transcribe mRNA from mononuclear phagocytes, specific IGF-II/M6P receptor cDNA was amplified and detected…
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Medical study · Medical research · 1999
Assessment of hazardous dust exposure by BAL and induced sputum
…T-lymphocyte subsets were analyzed by flow-activated cell sorter, and messenger RNA (mRNA) was transcribed by reverse transcriptase-polymerase chain reaction. Mineralogic particles were analyzed by scanning electron microscopy and polarizing light microscopy and quantified…
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Medical study · Medical research · 2012
A comparison of epidermal growth factor receptor expression in malignant peritoneal and pleural mesothelioma
…MM) specimens were subjected to IHC staining and FISH to evaluate the expression of EGFR protein and gene status. Overall positive IHC reaction was detected in 20/38 (53%) cases, in 11/22 (50%) pleural MM…
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Medical study · Medical research · 1992
Evidence of a role for mesothelial cell-derived interleukin 8 in the pathogenesis of asbestos-induced pleurisy in rabbits
…generated a probe for rabbit IL-8 mRNA by amplifying cDNA prepared from stimulated pleural mesothelial cells using the polymerase chain reaction (PCR) and primers based on homologous sequences in human and sheep IL-8 cDNAs…
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Medical study · Medical research · 2014
Targeting of the Hedgehog signal transduction pathway suppresses survival of malignant pleural mesothelioma cells in vitro
…annexinV/propidium iodide staining and flow cytometry. SMO small interfering RNA mediated a two- to more than fivefold reduction of SMO and Gli1 gene expression as determined by real-time quantitative reverse-transcriptase polymerase chain reaction…
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Medical study · Medical research · 2002
The presence of simian-virus 40 sequences in mesothelioma and mesothelial cells is associated with high levels of vascular endothelial growth factor
…detected in conditioned media of each of two MM cells that tested positive for SV40 by PCR amplification and Southern blot hybridization and for Tag transcript by reverse transcription- polymerase chain reaction (RT-PCR) and immunoprecipitation…
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Medical study · Medical research · 2001
Endothelial nitric oxide synthase is strongly expressed in malignant mesothelioma but does not associate with vascular density or the expression of VEGF, FLK1 or FLT1
…Two mesothelioma (M14K, M38K) and one non-neoplastic mesothelial cell line (MET-5A) were studied for eNOS mRNA expression by reverse transcriptase-polymerase chain reaction (RT-PCR). Vascular density was determined by staining the samples with…
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Medical study · Medical research · 2017
Tumor Suppressor microRNAs Contribute to the Regulation of PD-L1 Expression in Malignant Pleural Mesothelioma
…from samples from patients undergoing an operation (pleurectomy with or without decortication). MicroRNA expression was analyzed by reverse-transcriptase quantitative polymerase chain reaction. Regulation of PD-L1 expression in cell lines was assessed after transfection with…
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Medical study · Medical research · 2003
Expression of Clara cell secretory protein in the lungs of rats exposed to silicon carbide whisker in vivo
…1 month, 3 months and 6 months of recovery time. The expression of CCSP was observed by reverse transcriptase-polymerase chain reaction (RT-PCR), Western blot and immunostaining. Exposure to 10 mg of SiCW decreased in…
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Medical study · Medical research · 2012
Expression of the Ets transcription factor EHF in serous ovarian carcinoma effusions is a marker of poor survival
…using quantitative real-time polymerase chain reaction. Expression levels of EHF in ovarian carcinoma were additionally investigated for association with clinicopathologic parameters and survival. Quantitative real-time polymerase chain reaction analysis showed significantly higher expression of…
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Medical study · Medical research · 1997
Absence of p53 mutations in malignant mesotheliomas
…mesotheliomas. Thirteen malignant mesotheliomas collected over the preceding 12 yr and maintained in paraffin blocks were examined by polymerase chain reaction (PCR) followed by both single-strand conformation polymorphism (SSCP) and denaturing gradient-gel electrophoresis (DGGE)…
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Medical study · Medical research · 2014
Thermochemical destruction of asbestos-containing roofing slate and the feasibility of using recycled waste sulfuric acid
…chrysotile structure has revealed that the destruction of chrysotile by acid-digestion is greatly influenced by the reaction temperatures, showing a 80.3-fold increase in the reaction rate by raising the temperature by 30-100°C…
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Medical study · Medical research · 2014
Rat model demonstrates a high risk of tremolite but a low risk of anthophyllite for mesothelial carcinogenesis
…In half of the animals, repeated intraperitoneal injections of nitrilotriacetate (NTA), an iron chelator to promote Fenton reaction, were performed to evaluate the potential involvement of iron overload. Tremolite induced MM with a high incidence (96…
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Medical study · Medical research · 2012
Activated leukocyte cell-adhesion molecule (ALCAM) promotes malignant phenotypes of malignant mesothelioma
…MM cell lines. Activated leukocyte cell-adhesion molecule (ALCAM) expression in MM cell lines was analyzed with quantitative reverse transcription-polymerase chain reaction and Western blot analyses in 47 primary MM specimens with immunohistochemistry. ALCAM knockdown…
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Medical study · Medical research · 1988
Railroad workers with pleural plaques: II. Small airway dysfunction among asbestos-exposed workers
…groups the VTG:TLC ratio was elevated compared with controls. Increased VTG is a sign of small airways dysfunction. In asbestos-exposed subjects, it might be caused by an early peribronchial reaction to inhaled asbestos fibers.
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Medical study · Medical research · 2009
Association of transforming growth factor beta1 gene polymorphisms and asbestos-induced fibrosis and tumors
…Therefore, we examined an association between the 2 TGF-beta1 gene polymorphisms and asbestos-induced lung fibrosis and lung cancer. Detection of the 2 polymorphisms was performed by rapid capillary polymerase chain reaction, with melting curve…
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Medical study · Medical research · 1994
Genetic polymorphism for glutathione-S-transferase mu in asbestos cement workers
…findings, and lung function. Venous blood samples were obtained for the analysis of GSTM1 polymorphism by the polymerase chain reaction technique. Chest x ray films were classified according to the International Labour Organisation (ILO) 1980 classification…
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Medical study · Medical research · 2004
The pathology associated with therapeutic procedures in malignant mesothelioma
…biphasic pattern to the neoplasm. In more chronic cases, paucicellular fibrosis with a foreign body giant cell reaction is noted. The talc is polarizable and deposited in linear fashion within the tumour. In 2/28 (7…
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Medical study · Medical research · 2001
Expression and integrity of DNA topoisomerase II isoforms does not explain generic drug resistance in malignant mesothelioma
…Coupled with this, we have previously noted a common serological reaction to the beta isoform of topoisomerase II, suggesting that it may be aberrantly expressed in patients with mesothelioma. We assessed the expression of topoisomerase II…
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Medical study · Medical research · 2009
Survey and biological insights of pemetrexed-related therapeutic improvement in mesothelioma: The Nancy Centre of Biological Resources' Mesothelioma Cohort
…France (1989-2007). Blood or paired tumoral and normal samples were collected from the last 210 French patients to study the TCII single nucleotide polymorphisms at the codon 259 (quantitative polymerase chain reaction). Results were compared…