85 results for “Reaction Department”, page 3 of 5
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Medical study · Medical research · 2009
Association of transforming growth factor beta1 gene polymorphisms and asbestos-induced fibrosis and tumors
…Therefore, we examined an association between the 2 TGF-beta1 gene polymorphisms and asbestos-induced lung fibrosis and lung cancer. Detection of the 2 polymorphisms was performed by rapid capillary polymerase chain reaction, with melting curve…
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Medical study · Medical research · 2014
Expression profiling stratifies mesothelioma tumors and signifies deregulation of spindle checkpoint pathway and microtubule network with therapeutic implications
…We also carried out whole transcriptomic sequence (RNA-seq) analysis on eight tumor specimens. Taqman-based quantitative Reverse-transcription polymerase chain reaction (qRT-PCR), western analysis and immunohistochemistry (IHC) analysis of mitotic arrest deficient-like 1 …
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Medical study · Medical research · 2013
Expression and role of GLUT-1, MCT-1, and MCT-4 in malignant pleural mesothelioma
…MPM (n = 35) and RMH (n = 20) specimens by immunohistochemistry and in frozen tissue, and MPM cell lines, by real-time reverse transcription-polymerase chain reaction and western blot analysis. GLUT-1, MCT-1…
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Medical study · Medical research · 2011
miR-1254 and miR-574-5p: serum-based microRNA biomarkers for early-stage non-small cell lung cancer
…with early-stage NSCLC). Quantitative polymerase chain reaction (qPCR) was used to validate the profiling results in the discovery set and in a validation set of 31 controls and 22 patients with early-stage NSCLC. Additionally…
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Medical study · Medical research · 2012
Iron overload signature in chrysotile-induced malignant mesothelioma
…closely associated with iron overload: repeated administration of an iron chelator, nitrilotriacetic acid, which promotes the Fenton reaction, significantly reduced the period required for carcinogenesis; massive iron deposition was found in the peritoneal organs with high…
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Medical study · Medical research · 1996
Lung cancer risk in relation to the CYP2E1 Rsa I genetic polymorphism among African-Americans and Caucasians in Los Angeles County
…Samples of white blood cell DNA sufficient for determination of the CYP2E1 Rsa I genotype by a polymerase chain reaction-based assay were obtained from 341 cases and 706 controls with data on lifetime smoking history…
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Medical study · Medical research · 2013
ZIC1 is silenced and has tumor suppressor function in malignant pleural mesothelioma
…cell growth and apoptosis, was investigated in MPM cell lines and tumors. ZIC1 expression and promoter methylation were evaluated in MPM cell lines and tumor samples by quantitative polymerase chain reaction (PCR), Combined Bisulfite Restriction Analysis…
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Medical study · Medical research · 2002
Aberrant methylation of the CDKN2a/p16INK4a gene promoter region in preinvasive bronchial lesions: a prospective study in high-risk patients without invasive cancer
…the CDKN2A gene. Methylation-specific polymerase chain reaction was performed on DNA extracted from pure bronchial cell populations derived from biopsies and detection of p16 protein was studied by immunohistochemistry on contiguous parallel biopsies. Aberrant methylation…
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Medical study · Medical research · 2013
Differential expression of extracellular matrix constituents and cell adhesion molecules between malignant pleural mesothelioma and mesothelial hyperplasia
…carcinogenesis. We analyzed a panel of 84 genes involved in extracellular matrix remodeling and cell adhesion by polymerase chain reaction (PCR) array in 15 samples of epithelioid mesothelioma and 10 samples of reactive mesothelial hyperplasia (MH…
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Medical study · Medical research · 2013
Overexpression of EPH receptor B2 in malignant mesothelioma correlates with oncogenic behavior
…we investigated EPHB2 in MM tumors compared with normal mesothelium. EPHB2 and downstream target expression were evaluated using reverse-transcriptase polymerase chain reaction and immunoblotting methods. The biological significance of EPHB2 in MM was evaluated using…
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Medical study · Medical research · 2014
Epidermal growth factor receptor mutations in 510 Finnish non--small-cell lung cancer patients
…embedded, formalin-fixed tumor material using the following real-time polymerase chain reaction-based kits: Therascreen EGFR PCR Kit and cobas EGFR Mutation Test. EGFR mutation frequency was 11.4% and all positive cases were adenocarcinomas…
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Medical study · Medical research · 2001
Atypical carcinoid presenting as mesothelioma
…of the lung. In one case, pleural spread of tumor was seen while in the other an extensive fibrotic pleural reaction existed. To our knowledge these cases represent the first examples of atypical carcinoid causing pseudomesothelioma.
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Medical study · Medical research · 2005
The Human mineral dust-induced gene, mdig, is a cell growth regulating gene associated with lung cancer
…genes that are regulated by mineral dusts. Using a differential display reverse transcription polymerase chain reaction technique and mRNAs of alveolar macrophages from both normal individuals and coal miners, we identified a novel mineral dust-induced…
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Medical study · Medical research · 1983
Keratin proteins and carcinoembryonic antigen in lung carcinoma: an immunoperoxidase study of fifty-four cases, with ultrastructural correlations
…all squamous cell carcinomas. This reaction was particularly helpful in identifying squamous differentiation in poorly differentiated areas and in cases in which tonofilaments appeared to be sparse or absent on electron microscopy. Weak focal staining for…
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Medical study · Medical research · 2002
E-cadherin, E-selectin and vascular cell adhesion molecule: immunohistochemical markers for differentiation between mesothelioma and metastatic pulmonary adenocarcinoma?
…staining patterns for VCAM and E-selectin, there were significant differences in the expression of E-cadherin: while nearly all adenocarcinomas stained positively, there was almost no staining reaction of the mesotheliomas. Therefore, E-cadherin -- in…
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Medical study · Medical research · 2001
Can semi-automated image cytometry on induced sputum become a screening tool for lung cancer? Evaluation of quantitative semi-automated sputum cytometry on radon- and uranium-exposed workers
…workers resected for lung cancer, and 200 controls (50% smokers), were stained using the Papanicolaou (Pap) method and the Feulgen reaction with thionin. Cytometry was performed using the Cyto-Savant automated system. Atypical nuclei were found…
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Medical study · Medical research · 2013
miR-1 induces growth arrest and apoptosis in malignant mesothelioma
…comparators. To confirm microarray data, we used real-time quantitative polymerase chain reaction. Representative cell lines H513 and H2052 were used in functional analyses of miR-1. In addition to several novel MPM-associated microRNAs, we…
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Medical study · Medical research · 2010
Aberrant promoter methylation of WIF-1 and SFRP1, 2, 4 genes in mesothelioma
…We also analyzed WIF-1 and SFRPs promoter methylations in 46 mesothelioma tissues, 8 mesothelioma cell lines by methylation-specific polymerase chain reaction (MSP). WIF-1 promoter methylation was observed in 34 of 46 mesothelioma tissues…
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Medical study · Medical research · 2019
Proton Beam Therapy and Immune Checkpoint Inhibitors in Malignant Pleural Mesothelioma
…and a biopsy showed alveolar injury. Corticosteroids led to rapid symptom resolution. The authors hypothesized that proton therapy caused immune "priming" and a later "recall" reaction. They concluded that the combination, particularly with very large treatment…
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Medical study · Medical research · 2000
HBME-1, MOC-31, WT1 and calretinin: an assessment of recently described markers for mesothelioma and adenocarcinoma
… all reactive pleuras were negative. The reaction with Leu-M1 was graded as equivocal in 25% of the adenocarcinomas. All 24 of the autopsy cases of mesothelioma were negative for WT1 and in many operative specimens…