223 results for “Reaction Department”, page 6 of 12
-
Medical study · Medical research · 2012
Iron overload signature in chrysotile-induced malignant mesothelioma
…closely associated with iron overload: repeated administration of an iron chelator, nitrilotriacetic acid, which promotes the Fenton reaction, significantly reduced the period required for carcinogenesis; massive iron deposition was found in the peritoneal organs with high…
-
Medical study · Medical research · 1985
Immunohistochemical staining for vimentin and keratin in malignant mesothelioma
…were negative. In the mesotheliomas that did stain, both epithelial and spindled elements gave a positive reaction. Three alcohol-fixed lung cancers and two blocks of alcohol-fixed pleura failed to stain for vimentin. By contrast…
-
Medical study · Medical research · 2013
No association between simian virus 40 and diffuse malignant mesothelioma of the pleura in Iranian patients: a molecular and epidemiologic case-control study of 60 patients
…analysis. We related the patients with diffuse malignant mesothelioma to possible sites of exposure to asbestos. None of the DMMs nor any patient in the control group had SV40 genome on polymerase chain reaction (PCR). All…
-
Medical study · Medical research · 2013
ZIC1 is silenced and has tumor suppressor function in malignant pleural mesothelioma
…cell growth and apoptosis, was investigated in MPM cell lines and tumors. ZIC1 expression and promoter methylation were evaluated in MPM cell lines and tumor samples by quantitative polymerase chain reaction (PCR), Combined Bisulfite Restriction Analysis…
-
Medical study · Medical research · 2000
'Mucin-positive' epithelial mesothelioma of the peritoneum: an unusual diagnostic pitfall
…crystals. Repeat histochemical analysis following hyaluronidase pretreatment revealed a significantly diminished diastase-resistant periodic acid-Schiff reaction. This case of primary peritoneal 'mucin-positive' epithelial mesothelioma demonstrates morphological and histochemical mimicry with diffuse gastric adenocarcinoma. A…
-
Medical study · Medical research · 2013
Reduced folate carrier and folylpolyglutamate synthetase, but not thymidylate synthase predict survival in pemetrexed-treated patients suffering from malignant pleural mesothelioma
…AICARFT, RFC, and FPGS levels in MPM and associations with clinical benefit from pemetrexed treatment. Samples from 63 patients were tested using immunohistochemistry (IHC) and quantitative polymerase chain reaction(qPCR) for expression levels of TS, GARFT…
-
Medical study · Medical research · 1996
The calcium binding protein calretinin is a selective marker for malignant pleural mesotheliomas of the epithelial type
…adenomatous or solid) and by the epithelial component of the mixed tumours. The immunohistochemical reaction is specific and reproducible. The tissues of the pulmonary parenchyma and of the pleura are negative for calretinin except for the…
-
Medical study · Medical research · 1992
A novel biotinylated probe specific for hyaluronate. Its diagnostic value in diffuse malignant mesothelioma
…staining for hyaluronate. Furthermore, the staining reaction was classed as moderate or greater in 26 mesotheliomas (79%), thus suggesting the utility of this probe in the differential diagnosis of malignant mesothelioma versus adenocarcinoma. We conclude that…
-
Medical study · Medical research · 1998
Cytokine gene therapy or infusion as treatment for solid human cancer
…excretion was detectable. At each cycle vaccinia-IL-2 mRNA (SQ [semi-quantitative] reverse transcription polymerase chain reaction) was maximal 24-72 h following injection reduced at 8 days and faded by 21 days. No tumor…
-
Medical study · Medical research · 2011
Expression and regulation of B7-H3 immunoregulatory receptor, in human mesothelial and mesothelioma cells: immunotherapeutic implications
…agents in modulating levels of B7-H3 expression in MM cells was also studied. Reverse transcriptase-polymerase chain reaction (RT-PCR) demonstrated that B7-H3 mRNA was consistently detectable in mesothelial and MM cells investigated; however…
-
Medical study · Medical research · 2005
Identification of novel candidate oncogenes and tumor suppressors in malignant pleural mesothelioma using large-scale transcriptional profiling
…normal and tumor tissues, and tumors with different morphologies. Microarray gene expression data were confirmed using quantitative reverse transcriptase-polymerase chain reaction and protein analysis for three novel candidate oncogenes (NME2, CRI1, and PDGFC) and one…
-
Medical study · Medical research · 2013
Differential expression of extracellular matrix constituents and cell adhesion molecules between malignant pleural mesothelioma and mesothelial hyperplasia
…carcinogenesis. We analyzed a panel of 84 genes involved in extracellular matrix remodeling and cell adhesion by polymerase chain reaction (PCR) array in 15 samples of epithelioid mesothelioma and 10 samples of reactive mesothelial hyperplasia (MH…
-
Medical study · Medical research · 1983
Exposure to asbestos: psychological responses of mesothelioma patients
…to evaluate asbestos exposure, acquisition of increased risk information, and retrospective reporting of cognitive and behavioral reactions (particularly smoking behavior) to risk information. Twenty-eight patients (74%) had direct occupational contact with asbestos, and six patients…
-
Medical study · Medical research · 2013
Overexpression of EPH receptor B2 in malignant mesothelioma correlates with oncogenic behavior
…we investigated EPHB2 in MM tumors compared with normal mesothelium. EPHB2 and downstream target expression were evaluated using reverse-transcriptase polymerase chain reaction and immunoblotting methods. The biological significance of EPHB2 in MM was evaluated using…
-
Medical study · Medical research · 2011
A₃ receptors are overexpressed in pleura from patients with mesothelioma and reduce cell growth via Akt/nuclear factor-κB pathway
…between adenosine and MM, the presence and functionality of ARs were explored in human healthy mesothelial cells (HMC) and in malignant mesothelioma cells (MMC). ARs were analyzed by using reverse transcriptase-polymerase chain reaction, Western blotting…
-
Medical study · Medical research · 2010
No implication of Simian virus 40 in pathogenesis of malignant pleural mesothelioma in Slovenia
…antibody against Simian virus 40 (Pab280). SV40-expressing Wi-38 cells were used as positive control in both PCR and immunohistochemistry. In real-time polymerase chain reaction analyses, only 4 samples gave products with primer pairs…
-
Medical study · Medical research · 2011
MicroRNAs dysregulation in human malignant pleural mesothelioma
…with microarray approach. These results were confirmed by real-time quantitative reverse-transcriptase polymerase chain reaction and Western blotting. A comparative analysis of miRNA expression in MPM and HMCs was carried out. Microarray profiling showed different…
-
Medical study · Medical research · 2011
EphrinA1 inhibits malignant mesothelioma tumor growth via let-7 microRNA-mediated repression of the RAS oncogene
…repression of RAS proto-oncogene and the attenuation of MM tumor growth. The expression of miRNAs was determined by reverse transcription-quantitative polymerase chain reaction and in situ hybridization. RAS expression was determined by q-PCR…
-
Medical study · Medical research · 2013
Metabolic response to pegylated arginine deiminase in mesothelioma with promoter methylation of argininosuccinate synthetase
…1 (ASS1). Immunohistochemistry showed his tumor was ASS1-deficient, and methylation-specific polymerase chain reaction confirmed ASS1 promoter methylation. He was randomly assigned to 320 IU/m2/wk of ADI-PEG20 by intramuscular injection. After 3…
-
Medical study · Medical research · 2001
Atypical carcinoid presenting as mesothelioma
…of the lung. In one case, pleural spread of tumor was seen while in the other an extensive fibrotic pleural reaction existed. To our knowledge these cases represent the first examples of atypical carcinoid causing pseudomesothelioma.