Poly (3-hydroxybutyrate-co-3-hydroxyvalerate)/fibrinogen/bredigite nanofibrous membranes and their integration with osteoblasts for guided bone regeneration

Our plain-English summary of Poly (3-hydroxybutyrate-co-3-hydroxyvalerate)/fibrinogen/bredigite nanofibrous membranes and their integration with osteoblasts for guided bone regeneration, by M. Kouhi, V. Jayarama Reddy, M. Fathi, et al (Journal of Biomedical Materials Research. Part a, 2019).

The study

• Authors: M. Kouhi, V. Jayarama Reddy, M. Fathi, M. Shamanian, A. Valipouri, S. Ramakrishna
• Journal: Journal of Biomedical Materials Research. Part a
• Published: 2019;107(6):1154-1165
• Cited by: 25 articles, as counted by NIH iCite
• Read it: PubMed · Publisher

Abstract

Guided bone regeneration (GBR) has been established to be an effective method for the repair of defective tissues, which is based on isolating bone defects with a barrier membrane for faster tissue reconstruction. The aim of the present study is to develop poly (hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)/fibrinogen (FG)/bredigite (BR) membranes with applicability in GBR. BR nanoparticles were synthesized through a sol-gel method and characterized using transmission electron microscopy and X-ray diffractometer. PHBV, PHBV/FG, and PHBV/FG/BR membranes were fabricated using electrospinning and characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, contact angle, pore size, thermogravimetric analysis and tensile strength. The electrospun PHBV, PHBV/FG, and PHBV/FG/BR nanofibers were successfully obtained with the mean diameter ranging 240-410 nm. The results showed that Young's modulus and ultimate strength of the PHBV membrane reduced upon blending with FG and increased by further incorporation of BR nanoparticles, Moreover hydrophilicity of the PHBV membrane improved on addition of FG and BR. The in vitro degradation assay demonstrated that incorporation of FG and BR into PHBV matrix increased its hydrolytic degradation. Cell-membrane interactions were studied by culturing human fetal osteoblast cells on the fabricated membrane. According to the obtained results, osteoblasts seeded on PHBV/FG/BR displayed higher cell adhesion and proliferation compared to PHBV and PHBV/FG membrane. Furthermore, alkaline phosphatase activity and alizarin red-s staining indicated enhanced osteogenic differentiation and mineralization of cells on PHBV/FG/BR membranes. The results demonstrated that developed electrospun PHBV/FG/BR nanofibrous mats have desired potential as a barrier membrane for guided bone tissue engineering. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1154-1165, 2019.

© 2019 Wiley Periodicals, Inc.

In plain English

This article does not concern asbestos. It is a laboratory study of materials for guided bone regeneration, a technique that uses a thin barrier membrane over a bone defect. The authors made electrospun nanofiber membranes from PHBV, a bacterially produced polyester, alone, blended with fibrinogen (a blood protein), and blended with fibrinogen plus bredigite nanoparticles (a calcium magnesium silicate ceramic). They tested the membranes' physical properties and grew human fetal osteoblast (bone-forming) cells on them in lab dishes. No people or animals were studied. Adding fibrinogen made the membranes wetter and weaker. Adding bredigite as well made them stronger. Both additions made the membranes break down faster in a salt solution. By day 15, cells on the three-part membrane had grown 61.1% more than on plain PHBV and 14.5% more than on PHBV with fibrinogen. They also showed more mineral deposition. The authors concluded that the PHBV, fibrinogen and bredigite membranes have great potential for guided bone tissue engineering.

We wrote this plain-English summary from the full article with the help of AI, and a second AI model checked each sentence against the article. It is not the authors' wording; the abstract and the article are their own account.

What the study did and found

• Design: Laboratory (in vitro) materials study: membranes were fabricated and characterized, then tested with cultured cells
• Who was studied: Human fetal osteoblast (hFob) cells obtained from the American Type Culture Collection, grown on the membranes in culture; cells grown on tissue culture plastic served as the control
• Exposure: No asbestos exposure was studied. The materials tested were electrospun membranes of PHBV alone, PHBV blended with fibrinogen (80/20 by weight), and PHBV/fibrinogen with bredigite nanoparticles (10 wt % of total content)
• Main results:
  • Water contact angle fell from 129 degrees on PHBV membranes to 83 degrees on PHBV/fibrinogen membranes (p less than or equal to 0.05), meaning the surface became more wettable. PHBV/fibrinogen/bredigite membranes measured 76 degrees.
  • PHBV/fibrinogen/bredigite membranes had a Young's modulus of 121 MPa and a tensile strength of 5.22 MPa. Both were significantly higher than for the PHBV/fibrinogen membranes (83.3 MPa and 3.68 MPa) and the PHBV membranes (106.7 MPa and 4.4 MPa).
  • After 28 days in phosphate buffer, pure PHBV membranes lost 3.2% of their weight. The PHBV/fibrinogen membranes lost 28% and the PHBV/fibrinogen/bredigite membranes lost 33%.
  • On day 15, osteoblast proliferation on PHBV/fibrinogen/bredigite membranes was 61.1% higher than on PHBV membranes and 14.5% higher than on PHBV/fibrinogen membranes.
  • Alkaline phosphatase activity, a marker of osteoblast maturation, was significantly higher on PHBV/fibrinogen/bredigite membranes than on PHBV/fibrinogen membranes on day 15.
  • After 15 days, the alizarin red mineral stain eluted from PHBV/fibrinogen/bredigite membranes was 20% higher than from PHBV/fibrinogen membranes and 56% higher than from PHBV membranes.
• The authors' conclusion: The authors concluded that the electrospun PHBV/fibrinogen/bredigite nanofibrous membranes have great potential for guided bone tissue engineering applications.

Topics

The National Library of Medicine indexes this study under subjects our site covers: Tremolite.

Medical terms in this study, explained in our Lexicon: Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Thermogravimetric Analysis, Tensile Strength.

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