558 results for “Reaction Department”, page 14 of 28
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Medical study · Medical research · 2009
A novel decomposition technique of friable asbestos by CHClF2-decomposed acidic gas
…for 30 min, respectively, by CHClF(2)-decomposed acidic gas. The observation of the reaction products by phase-contrast microscopy (PCM) and scanning electron microscopy (SEM) confirmed that the resulting products did not contain any asbestos.
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Medical study · Medical research · 2014
Expression profiling stratifies mesothelioma tumors and signifies deregulation of spindle checkpoint pathway and microtubule network with therapeutic implications
…We also carried out whole transcriptomic sequence (RNA-seq) analysis on eight tumor specimens. Taqman-based quantitative Reverse-transcription polymerase chain reaction (qRT-PCR), western analysis and immunohistochemistry (IHC) analysis of mitotic arrest deficient-like 1 …
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Medical study · Medical research · 1998
Human mesotheliomas contain the simian virus-40 regulatory region and large tumor antigen DNA sequences
…40-induced hamster mesothelioma tumor H9A. Polymerase chain reaction primers were used to amplify various simian virus 40 large T-antigen regions including a 105-base pair amino terminus fragment, a 281-base pair carboxyl terminus…
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Medical study · Medical research · 1991
Preparation of radiolabeled bioactive asbestos fibers
…were radiolabeled successfully. Mild reaction conditions and short reaction times were found under which approximately 90% of the added 68Ge and 68Ga bound to the fibers. The radiolabel was retained even after washing the fibers extensively…
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Medical study · Medical research · 2007
Change of heme oxygenase-1 expression in lung injury induced by chrysotile asbestos in vivo and in vitro
…and 6 mo of recovery time. The expression of HO-1 was observed by Western blot analysis, reverse-transcription polymerase chain reaction, and immunostaining. Protein levels of HO-1 increased at from 3 days to 6…
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Medical study · Medical research · 1998
Asbestos fibers and interleukin-1 upregulate the formation of reactive nitrogen species in rat pleural mesothelial cells
…polymerase chain reaction (RT-PCR), increased progressively from 2 to 12 h in IL-1beta-containing cultures. Nitrite (NO2-), the stable oxidation product of .NO in mesothelial cell conditioned medium, was assayed through the Griess reaction…
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Medical study · Medical research · 2009
Tobacco smoke: involvement of reactive oxygen species and stable free radicals in mechanisms of oxidative damage, carcinogenesis and synergistic effects with other respirable particles
…8-hydroxy-2'-deoxyguanosine (a biomarker for carcinogenesis). Also, we observed synergistic effects in the generation of HO(), through the Fenton reaction, with environmental respirable particles (asbestos fibres, coal dust, etc.) and ambient particulate matter (PM)…
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Medical study · Medical research · 2013
Expression and role of GLUT-1, MCT-1, and MCT-4 in malignant pleural mesothelioma
…MPM (n = 35) and RMH (n = 20) specimens by immunohistochemistry and in frozen tissue, and MPM cell lines, by real-time reverse transcription-polymerase chain reaction and western blot analysis. GLUT-1, MCT-1…
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Medical study · Medical research · 1995
Chrysotile asbestos stimulates platelet-derived growth factor-AA production by rat lung fibroblasts in vitro: evidence for an autocrine loop
…However, message amplification using a reverse transcriptase polymerase chain reaction allowed detection of the B-chain message. A significant dose-dependent mitogenic effect of asbestos was found by using both a cell proliferation assay and nuclear…
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Medical study · Medical research · 2012
Extranodal marginal zone lymphoma of mucosa-associated lymphoid tissue type arising in the pleura with pleural fibrous plaques in a lathe worker
…pleura and diffuse infiltration of small- to medium-sized B lymphocytes. Polymerase chain reaction-based analysis detected monoclonal rearrangement of immunoglobulin heavy-chain genes. Histologic diagnosis was extranodal marginal zone lymphoma of mucosa-associated lymphoid tissue…
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Medical study · Medical research · 2011
miR-1254 and miR-574-5p: serum-based microRNA biomarkers for early-stage non-small cell lung cancer
…with early-stage NSCLC). Quantitative polymerase chain reaction (qPCR) was used to validate the profiling results in the discovery set and in a validation set of 31 controls and 22 patients with early-stage NSCLC. Additionally…
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Medical study · Medical research · 1999
Production of reactive oxygen intermediates by human macrophages exposed to soot particles and asbestos fibers and increase in NF-kappa B p50/p105 mRNA
…100 micrograms/10(6) cells) compared with control experiments (p < 0.01). Identical exposure conditions for BM resulted in a similar reaction pattern (maximum 2.2-fold increase in ROI release; p < 0.05)…
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Medical study · Medical research · 2008
In situ ESEM study of the thermal decomposition of chrysotile asbestos in view of safe recycling of the transformation product
…in cement-asbestos. It was found that the reaction kinetics of thermal transformation of chrysotile was highly slowed down in the presence of water vapor in the experimental chamber with respect to He. This was explained…
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Medical study · Medical research · 2013
RNA sequencing identifies fusion of the EWSR1 and YY1 genes in mesothelioma with t(14;22)(q32;q12)
…an EWSR1-YY1 fusion gene but not the reciprocal YY1-EWSR1 transcript. RT-polymerase chain reaction and sequencing showed that in both cases exon 8 of EWSR1 (nucleotide 1,139, accession number NM_013986 version 3…
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Medical study · Medical research · 2012
Iron overload signature in chrysotile-induced malignant mesothelioma
…closely associated with iron overload: repeated administration of an iron chelator, nitrilotriacetic acid, which promotes the Fenton reaction, significantly reduced the period required for carcinogenesis; massive iron deposition was found in the peritoneal organs with high…
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Medical study · Medical research · 2009
Rapid and easy method for monitoring oxidative stress markers in body fluids of patients with asbestos or silica-induced lung diseases
…method LC-ESI-MS/MS, where the selected reaction monitoring mode was used for its extremely high degree of selectivity and the stable-isotope-dilution assay for its high precision of quantification. The developed method was…
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Medical study · Medical research · 1996
Lung cancer risk in relation to the CYP2E1 Rsa I genetic polymorphism among African-Americans and Caucasians in Los Angeles County
…Samples of white blood cell DNA sufficient for determination of the CYP2E1 Rsa I genotype by a polymerase chain reaction-based assay were obtained from 341 cases and 706 controls with data on lifetime smoking history…
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Medical study · Medical research · 1985
Immunohistochemical staining for vimentin and keratin in malignant mesothelioma
…were negative. In the mesotheliomas that did stain, both epithelial and spindled elements gave a positive reaction. Three alcohol-fixed lung cancers and two blocks of alcohol-fixed pleura failed to stain for vimentin. By contrast…
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Medical study · Medical research · 1996
Comparative analysis of p16/CDKN2, p53 and ras gene alterations in human non-small cell lung cancers, with and without associated pulmonary asbestosis
…were examined for genetic abnormalities of p16/CDKN2, p53 and ras genes by single-strand conformation polymorphism analysis of polymerase chain reaction products (PCR-SSCP) and direct sequencing. Ten specimens were obtained from autopsies in which…
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Medical study · Medical research · 2013
No association between simian virus 40 and diffuse malignant mesothelioma of the pleura in Iranian patients: a molecular and epidemiologic case-control study of 60 patients
…analysis. We related the patients with diffuse malignant mesothelioma to possible sites of exposure to asbestos. None of the DMMs nor any patient in the control group had SV40 genome on polymerase chain reaction (PCR). All…