124 results for “Reaction Department”, page 3 of 7
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Medical study · Medical research · 2001
Expression and integrity of DNA topoisomerase II isoforms does not explain generic drug resistance in malignant mesothelioma
…Coupled with this, we have previously noted a common serological reaction to the beta isoform of topoisomerase II, suggesting that it may be aberrantly expressed in patients with mesothelioma. We assessed the expression of topoisomerase II…
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Medical study · Medical research · 2009
Survey and biological insights of pemetrexed-related therapeutic improvement in mesothelioma: The Nancy Centre of Biological Resources' Mesothelioma Cohort
…France (1989-2007). Blood or paired tumoral and normal samples were collected from the last 210 French patients to study the TCII single nucleotide polymorphisms at the codon 259 (quantitative polymerase chain reaction). Results were compared…
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Medical study · Medical research · 2009
A novel decomposition technique of friable asbestos by CHClF2-decomposed acidic gas
…for 30 min, respectively, by CHClF(2)-decomposed acidic gas. The observation of the reaction products by phase-contrast microscopy (PCM) and scanning electron microscopy (SEM) confirmed that the resulting products did not contain any asbestos.
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Medical study · Medical research · 2007
Change of heme oxygenase-1 expression in lung injury induced by chrysotile asbestos in vivo and in vitro
…and 6 mo of recovery time. The expression of HO-1 was observed by Western blot analysis, reverse-transcription polymerase chain reaction, and immunostaining. Protein levels of HO-1 increased at from 3 days to 6…
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Medical study · Medical research · 2009
Tobacco smoke: involvement of reactive oxygen species and stable free radicals in mechanisms of oxidative damage, carcinogenesis and synergistic effects with other respirable particles
…8-hydroxy-2'-deoxyguanosine (a biomarker for carcinogenesis). Also, we observed synergistic effects in the generation of HO(), through the Fenton reaction, with environmental respirable particles (asbestos fibres, coal dust, etc.) and ambient particulate matter (PM)…
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Medical study · Medical research · 2008
In situ ESEM study of the thermal decomposition of chrysotile asbestos in view of safe recycling of the transformation product
…in cement-asbestos. It was found that the reaction kinetics of thermal transformation of chrysotile was highly slowed down in the presence of water vapor in the experimental chamber with respect to He. This was explained…
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Medical study · Medical research · 2009
Rapid and easy method for monitoring oxidative stress markers in body fluids of patients with asbestos or silica-induced lung diseases
…method LC-ESI-MS/MS, where the selected reaction monitoring mode was used for its extremely high degree of selectivity and the stable-isotope-dilution assay for its high precision of quantification. The developed method was…
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Medical study · Medical research · 2007
Asbestos-induced peribronchiolar cell proliferation and cytokine production are attenuated in lungs of protein kinase C-delta knockout mice
…decreased in PKCdelta-/- mice. At 9 days, microarray and quantitative reverse transcriptase-polymerase chain reaction analysis of lung tissues revealed increased mRNA levels of the profibrotic cytokine, IL-4, in asbestos-exposed wild-type mice but…
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Medical study · Medical research · 2000
'Mucin-positive' epithelial mesothelioma of the peritoneum: an unusual diagnostic pitfall
…crystals. Repeat histochemical analysis following hyaluronidase pretreatment revealed a significantly diminished diastase-resistant periodic acid-Schiff reaction. This case of primary peritoneal 'mucin-positive' epithelial mesothelioma demonstrates morphological and histochemical mimicry with diffuse gastric adenocarcinoma. A…
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Medical study · Medical research · 2002
Aberrant methylation of the CDKN2a/p16INK4a gene promoter region in preinvasive bronchial lesions: a prospective study in high-risk patients without invasive cancer
…the CDKN2A gene. Methylation-specific polymerase chain reaction was performed on DNA extracted from pure bronchial cell populations derived from biopsies and detection of p16 protein was studied by immunohistochemistry on contiguous parallel biopsies. Aberrant methylation…
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Medical study · Medical research · 2005
Identification of novel candidate oncogenes and tumor suppressors in malignant pleural mesothelioma using large-scale transcriptional profiling
…normal and tumor tissues, and tumors with different morphologies. Microarray gene expression data were confirmed using quantitative reverse transcriptase-polymerase chain reaction and protein analysis for three novel candidate oncogenes (NME2, CRI1, and PDGFC) and one…
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Medical study · Medical research · 2007
Beyond foreign-body-induced carcinogenesis: impact of reactive oxygen species derived from inflammatory cells in tumorigenic conversion and tumor progression
…of foreign-body-induced tumorigenesis. What is notable is that the foreign bodies themselves are unrelated to each other, whereas commonly underlying in them is to induce inflammatory reaction, especially stromal proliferation, where those exogenous materials…
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Medical study · Medical research · 2001
Changes in DNA 8-hydroxyguanine levels, 8-hydroxyguanine repair activity, and hOGG1 and hMTH1 mRNA expression in human lung alveolar epithelial cells induced by crocidolite asbestos
…and human MUtT homologue (hMTH1). This was done using a high-performance liquid chromatography system equipped with an electrochemical detector, endonuclease nicking assay and reverse transcription polymerase chain reaction, respectively. Crocidolite induced the formation of 8…
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Medical study · Medical research · 2001
Glypican-3 expression in Wilms tumor and hepatoblastoma
…and protein were extracted from Wilms tumor and hepatoblastoma tissue samples and GPC3 levels were measured in these extracts by Northern blotting, reverse transcription polymerase chain reaction, and immunoblotting. In contrast to published results with carcinomas…
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Medical study · Medical research · 2008
Characteristics and modifying factors of asbestos-induced oxidative DNA damage
…Natural iron chelators, such as citrate, adenosine 5'-triphosphate and guanosine 5'-triphosphate, did not inhibit this reaction. Second, we used time-lapse video microscopy to evaluate how cells cope with asbestos fibers. RAW264.7 cells…
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Medical study · Medical research · 2003
Comparative study of antioxidant properties and cytoprotective activity of flavonoids
…studied in a model photochemical system. Rate constants of the second order reaction obtained by competitive kinetics suggested flavonoids to be more effective scavengers of oxygen anion-radicals than ascorbic acid. By competitive replacement all flavonoids…
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Medical study · Medical research · 2002
Chemiluminescent detection of induced reactive oxygen metabolite production of human polymorphonuclear leucocytes by anthophyllite asbestos
…tyrosine kinase, which resides in the pathway to cause PMN activation. The strong ROM reaction of PMN by anthophyllite suggests that the surface characteristics of the fiber may participate in the pathogenic mechanisms of anthophyllite asbestos.
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Medical study · Medical research · 2009
Asbestos health hazard: a spectroscopic study of synthetic geoinspired Fe-doped chrysotile
…the Fe doping extent. Geoinspired synthetic chrysotile at different Fe doping extents has been obtained as unique phase by hydrothermal reaction in the presence or not of metallic Fe in the synthetic environment. The results highlight…
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Medical study · Medical research · 2002
Black spots of the parietal pleura: morphology and formal pathogenesis
…in close correlation to lymphatic channels and blood vessels. Their formal pathogenesis is characterized by a mild fibrosis and an inflammatory reaction to the incorporated foreign particles. The proliferation of connective tissue may result in the…
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Medical study · Medical research · 2009
Beta-carotene metabolites enhance inflammation-induced oxidative DNA damage in lung epithelial cells
…Moreover, BC and BCM were able to increase (.)OH formation from H(2)O(2) in the Fenton reaction (determined by electron spin resonance spectroscopy). Addition of Vit A and RA to lung epithelial cells that…